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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ABCA8 Polyclonal antibody

ABCA8 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 24351-1-AP

Synonyms

ABCA8, KIAA0822

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse brain tissue using ABCA8 Polyclonal antibody (24351-1-AP)

    WB analysis of mouse brain using 24351-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 24351-1-AP (ABCA8 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver tissue using ABCA8 Polyclonal antibody (24351-1-AP)

    IHC staining of human liver using 24351-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 24351-1-AP (ABCA8 Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using ABCA8 Polyclonal antibody (24351-1-AP)

    IHC staining of human liver using 24351-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 24351-1-AP (ABCA8 Antibody) at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using ABCA8 Polyclonal antibody (24351-1-AP)

    IHC staining of mouse brain using 24351-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 24351-1-AP (ABCA8 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using ABCA8 Polyclonal antibody (24351-1-AP)

    IHC staining of mouse brain using 24351-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 24351-1-AP (ABCA8 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inmouse brain tissue
Positive IHC detected inhuman liver tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 24351-1-AP is ABCA8 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ABCA8 fusion protein Ag18991 相同性解析による交差性が予測される生物種
Full Name ATP-binding cassette, sub-family A (ABC1), member 8
Calculated molecular weight 1581 aa, 179 kDa
Observed molecular weight 160-180 kDa
GenBank accession numberbc130280
Gene symbol ABCA8
Gene ID (NCBI) 10351
RRIDAB_2879504
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for ABCA8 antibody 24351-1-APDownload protocol
IHC protocol for ABCA8 antibody 24351-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle

Cancer Cell

Tumor cell-intrinsic epigenetic dysregulation shapes cancer-associated fibroblasts heterogeneity to metabolically support pancreatic cancer

Authors - Ningning Niu
View Article