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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ABCB4 Polyclonal antibody (27726-1-AP)

    WB analysis using 27726-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27726-1-AP (ABCB4 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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ABCB4 Polyclonal antibody

ABCB4 Polyclonal Antibody for WB, ELISA
Cat No. 27726-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, ELISA

Synonyms

ABC21, ABCB4, GBD1, MDR2, MDR2/3, MDR3, Multidrug resistance protein 3, P glycoprotein 3, PFIC 3, PGY3

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, MCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

27726-1-AP targets ABCB4 in WB, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ABCB4 fusion protein Ag26840 相同性解析による交差性が予測される生物種
Full Name ATP-binding cassette, sub-family B (MDR/TAP), member 4
Calculated molecular weight 1286 aa, 142 kDa
Observed molecular weight130-140 kDa
GenBank accession numberBC042531
Gene Symbol ABCB4
Gene ID (NCBI) 5244
RRIDAB_2918128
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP21439
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for ABCB4 antibody 27726-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols