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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ABCB5 Polyclonal antibody (20703-1-AP)

    WB analysis using 20703-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 20703-1-AP (ABCB5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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ABCB5 Polyclonal antibody

ABCB5 Polyclonal Antibody for WB, ELISA
Cat No. 20703-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

P-glycoprotein ABCB5, EC:7.6.2.2, ATP-binding cassette sub-family B member 5, ABCB5beta, ABCB5alpha

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inA431 cells, HT-29 cells, Jurkat cells, K-562 cells, THP-1 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

20703-1-AP targets ABCB5 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name ATP-binding cassette, sub-family B (MDR/TAP), member 5
Calculated molecular weight 90 kDa
Observed molecular weight 90-110 kDa
GenBank accession numberNM_178559
Gene Symbol ABCB5
Gene ID (NCBI) 340273
RRIDAB_3085616
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ2M3G0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ABCB5 is an 812 amino acid multi-pass membrane protein and belongs to the ATP-binding cassette (ABC) transporter superfamily of integral membrane proteins (PMID: 15760339). ABCB5 is an Energy-dependent efflux transporter responsible for decreased drug accumulation in multidrug-resistant cells(PMID: 15899824, 15205344). ABCB5 acts as a marker of stem-like cells (CSC) in several malignancies and is responsible for the resistance to doxorubicin of a subset of malignant melanomas (PubMed:24934811, 15760339). Alternate splicing results in multiple transcript variants.

Protocols

Product Specific Protocols
WB protocol for ABCB5 antibody 20703-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols