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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ABCB6 Polyclonal antibody

ABCB6 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 14996-1-AP

Synonyms

ABC, ABC14, ABCB6, EST45597, Mt ABC transporter 3, MTABC3, P glycoprotein related protein, PRP, umat

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse brain tissue using ABCB6 Polyclonal antibody (14996-1-AP)

    WB analysis of mouse brain using 14996-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 14996-1-AP (ABCB6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using ABCB6 Polyclonal antibody (14996-1-AP)

    WB analysis of mouse brain using 14996-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 14996-1-AP (ABCB6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using ABCB6 Polyclonal antibody (14996-1-AP)

    IHC staining of human breast cancer using 14996-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14996-1-AP (ABCB6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using ABCB6 Polyclonal antibody (14996-1-AP)

    IHC staining of human breast cancer using 14996-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14996-1-AP (ABCB6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using ABCB6 Polyclonal antibody (14996-1-AP)

    IHC staining of human ovary tumor using 14996-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 14996-1-AP (ABCB6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using ABCB6 Polyclonal antibody (14996-1-AP)

    IHC staining of human ovary tumor using 14996-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 14996-1-AP (ABCB6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using ABCB6 Polyclonal antibody (14996-1-AP)

    IHC staining of human breast cancer using 14996-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14996-1-AP (ABCB6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using ABCB6 Polyclonal antibody (14996-1-AP)

    IHC staining of human breast cancer using 14996-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14996-1-AP (ABCB6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inmouse brain tissue
Positive IHC detected inhuman breast cancer tissue, human ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14996-1-AP targets ABCB6 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ABCB6 fusion protein Ag6982 相同性解析による交差性が予測される生物種
Full Name ATP-binding cassette, sub-family B (MDR/TAP), member 6
Calculated molecular weight 94 kDa
Observed molecular weight 170 kDa
GenBank accession numberBC000559
Gene symbol ABCB6
Gene ID (NCBI) 10058
RRIDAB_2220828
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for ABCB6 antibody 14996-1-APDownload protocol
IHC protocol for ABCB6 antibody 14996-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols