Validation Data Gallery
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Tested Applications
| Positive WB detected in | HeLa cells, U-251 cells |
| Positive IF/ICC detected in | MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 3 publications below |
| IHC | See 2 publications below |
Product Information
51007-1-AP targets ABCB6 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | ABCB6 fusion protein Ag0461 相同性解析による交差性が予測される生物種 |
| Full Name | ATP-binding cassette, sub-family B (MDR/TAP), member 6 |
| Calculated molecular weight | 94 kDa |
| Observed molecular weight | 70-79 kDa |
| GenBank accession number | BC000559 |
| Gene Symbol | ABCB6 |
| Gene ID (NCBI) | 10058 |
| RRID | AB_2220830 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9NP58 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for ABCB6 antibody 51007-1-AP | Download protocol |
| IF protocol for ABCB6 antibody 51007-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Commun Signal The ferroptosis and iron-metabolism signature robustly predicts clinical diagnosis, prognosis and immune microenvironment for hepatocellular carcinoma. | ||
PLoS One Hepatitis C virus core protein triggers abnormal porphyrin metabolism in human hepatocellular carcinoma cells. | ||
Free Radic Biol Med Targeting ABCB6 with nitidine chloride inhibits PI3K/AKT signaling pathway to promote ferroptosis in multiple myeloma |