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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ABCC5 Recombinant antibody

ABCC5 Recombinant Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

241380G1

Cat no : 84204-5-RR

Synonyms

MRP5, MOAT C, EC:7.6.2.-, ATP-binding cassette sub-family C member 5, ABCC 5

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ABCC5 Recombinant antibody (84204-5-RR)

    WB analysis using 84204-5-RR

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 84204-5-RR (ABCC5 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A549 cells using ABCC5 Recombinant antibody (84204-5-RR)

    WB analysis of A549 using 84204-5-RR

    A549 cells were subjected to SDS PAGE followed by western blot with 84204-5-RR (ABCC5 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using ABCC5 Recombinant antibody (84204-5-RR)

    IHC staining of human ovary cancer using 84204-5-RR

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 84204-5-RR (ABCC5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using ABCC5 Recombinant antibody (84204-5-RR)

    IHC staining of human ovary cancer using 84204-5-RR

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 84204-5-RR (ABCC5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of PC-3 cells using ABCC5 Recombinant antibody (84204-5-RR)

    IF Staining of PC-3 using 84204-5-RR

    Immunofluorescent analysis of (4% PFA) fixed PC-3 cells using ABCC5 antibody (84204-5-RR, Clone: 241380G1 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 84204-5-RR

    Affinity and Kinetic Characterization of 84204-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84204-5-RR against Human ABCC5 were performed. The affinity constant is 2.18 nM.

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Tested Applications

Positive WB detected inMCF-7 cells, A549 cells, HepG2 cells
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inPC-3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 84204-5-RR is ABCC5 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen ABCC5 fusion protein Ag31680 相同性解析による交差性が予測される生物種
Full Name ATP-binding cassette, sub-family C (CFTR/MRP), member 5
Calculated molecular weight 161 kDa
Observed molecular weight161-200 kDa
GenBank accession numberNM_005688
Gene symbol ABCC5
Gene ID (NCBI) 10057
RRIDAB_3671760
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ABCC5, also named MOAT-C, pABC11, SMRP, and MRP5, belongs to the ABC transporter superfamily, ABCC family, and Conjugate transporter (TC 3.A.1.208) subfamily. ABCC5 acts as a multispecific organic anion pump that can transport nucleotide analogs. ABCC5 functions in the cellular export of its substrate, cyclic nucleotides. This export contributes to the degradation of phosphodiesterases and possibly an elimination pathway for cyclic nucleotides. Studies show that ABCC5 provides resistance to thiopurine anticancer drugs, 6-mercaptopurine and thioguanine, and the anti-HIV drug 9-(2-phosphonylmethoxyethyl) adenine. ABCC5 may be involved in resistance to thiopurines in acute lymphoblastic leukemia and antiretroviral nucleoside analogs in HIV-infected patients. Alternative splicing of this gene has been detected; however, the complete sequence and translation initiation site are unclear. Since it is glycosylated, the apparent molecular weight of ABCC5 could be variable, ranging from 161 kDa to 200 kDa (PMID: 10893247; PMID: 15897250; PMID: 31338999).

Protocols

Product Specific Protocols
WB protocol for ABCC5 antibody 84204-5-RRDownload protocol
IHC protocol for ABCC5 antibody 84204-5-RRDownload protocol
IF protocol for ABCC5 antibody 84204-5-RRDownload protocol
Standard Protocols
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