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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SUR2/ABCC9 Polyclonal antibody (13599-1-AP)

    WB analysis using 13599-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13599-1-AP (SUR2/ABCC9 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using SUR2/ABCC9 Polyclonal antibody (13599-1-AP)

    WB analysis using 13599-1-AP

    mouse heart were subjected to SDS PAGE followed by western blot with 13599-1-AP (SUR2/ABCC9 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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SUR2/ABCC9 Polyclonal antibody

SUR2/ABCC9 Polyclonal Antibody for WB, ELISA
Cat No. 13599-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

ABC37, ABCC9, CMD1O, Sulfonylurea receptor 2, SUR2, SUR2/ABCC9

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse heart tissue, mouse skeletal muscle tissue, unboiled mouse heart tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13599-1-AP targets SUR2/ABCC9 in WB, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SUR2/ABCC9 fusion protein Ag4536 相同性解析による交差性が予測される生物種
Full Name ATP-binding cassette, sub-family C (CFTR/MRP), member 9
Calculated molecular weight 1549 aa, 174 kDa
Observed molecular weight174 kDa
GenBank accession numberBC033804
Gene Symbol SUR2/ABCC9
Gene ID (NCBI) 10060
RRIDAB_3085419
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO60706
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ABCC9, also known as SUR2, belongs to the ABC transporter superfamily. ABCC9 is a subunit of ATP-sensitive potassium channels (KATP), forming cardiac and smooth muscle-type KATP channels with KCNJ11. KCNJ11 forms the channel pore while ABCC9 is required for activation and regulation (PMID: 9831708). ABCC9 is thought to form ATP-sensitive potassium channels in cardiac, skeletal, vascular, and non-vascular smooth muscle. Defects in ABCC9 cause dilated cardiomyopathy 10 and familial atrial fibrillation type 12 (ATFB12)(PMID: 15034580,17245405).

Protocols

Product Specific Protocols
WB protocol for SUR2/ABCC9 antibody 13599-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols