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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ABCD1 Polyclonal antibody

ABCD1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 11159-1-AP

Synonyms

EC:3.1.2.-, ATP-binding cassette sub-family D member 1, ATP binding cassette sub family D member 1, ALD, ABCD 1

Validation Data Gallery

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  • Western Blot (WB) analysis of human brain tissue using ABCD1 Polyclonal antibody (11159-1-AP)

    WB analysis of human brain using 11159-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 11159-1-AP (ABCD1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse liver tissue using ABCD1 Polyclonal antibody (11159-1-AP)

    WB analysis of mouse liver using 11159-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 11159-1-AP (ABCD1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using ABCD1 Polyclonal antibody (11159-1-AP)

    WB analysis of HeLa using 11159-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 11159-1-AP (ABCD1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse liver tissue using ABCD1 Polyclonal antibody (11159-1-AP)

    IP experiment of mouse liver using 11159-1-AP

    IP result of anti-ABCD1 (IP:11159-1-AP, 4ug; Detection:11159-1-AP 1:500) with mouse liver tissue lysate 5000ug.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using ABCD1 Polyclonal antibody (11159-1-AP)

    IHC staining of human lung cancer using 11159-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer using 11159-1-AP (ABCD1 antibody) at dilution of 1:50 (under 10x lens).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using ABCD1 Polyclonal antibody (11159-1-AP)

    IF Staining of HeLa using 11159-1-AP

    Immunofluorescent analysis of HeLa cells using 11159-1-AP (ABCD1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inhuman brain tissue, HeLa cells, mouse liver tissue
Positive IP detected inmouse liver tissue
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11159-1-AP targets ABCD1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ABCD1 fusion protein Ag1635 相同性解析による交差性が予測される生物種
Full Name ATP-binding cassette, sub-family D (ALD), member 1
Calculated molecular weight 745 aa, 83 kDa
Observed molecular weight 75 kDa
GenBank accession numberBC025358
Gene symbol ABCD1
Gene ID (NCBI) 215
RRIDAB_2219775
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ABCD1 (also known as ALDP) is a member of the ATP-binding cassette (ABC) transporter superfamily which functions as transporter for a wide variety of substrates. It localizes to the peroxisomal membrane. The exact function is not clear so far. Various mutations of ABCD1 cause X-linked adrenoleukodystrophy (X-ALD), an inherited neurodegenerative disease affecting the nervous system white matter and adrenal cortex.

Protocols

Product Specific Protocols
WB protocol for ABCD1 antibody 11159-1-APDownload protocol
IHC protocol for ABCD1 antibody 11159-1-APDownload protocol
IF protocol for ABCD1 antibody 11159-1-APDownload protocol
IP protocol for ABCD1 antibody 11159-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cancers (Basel)

Prostate Cancer Proliferation Is Affected by the Subcellular Localization of MCT2 and Accompanied by Significant Peroxisomal Alterations.

Authors - Isabel Valença
View Article
mouseIF

J Biomed Res

Germ cell-specific deletion of Pex3 reveals essential roles of PEX3-dependent peroxisomes in spermiogenesis

Authors - Yejin Yao
View Article