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Species Reactivity

Species Reactivity

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  • Featured Product
  • KD/KO Validated

ABCD1 Polyclonal antibody

ABCD1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugate

Unconjugated

Cat no : 18138-1-AP

Synonyms

EC:3.1.2.-, ATP-binding cassette sub-family D member 1, ATP binding cassette sub family D member 1, ALD, ABCD 1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ABCD1 Polyclonal antibody (18138-1-AP)

    WB analysis using 18138-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 18138-1-AP (ABCD1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using ABCD1 Polyclonal antibody (18138-1-AP)

    WB analysis of HeLa using 18138-1-AP

    WB result of ABCD1 antibody (18138-1-AP, 1:600) with si-Control and si-ABCD1 transfected HeLa cells..

  • Western Blot (WB) analysis of HepG2 cells using ABCD1 Polyclonal antibody (18138-1-AP)

    WB analysis of HepG2 using 18138-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 18138-1-AP (ABCD1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using ABCD1 Polyclonal antibody (18138-1-AP)

    WB analysis of HeLa using 18138-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 18138-1-AP (ABCD1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using ABCD1 Polyclonal antibody (18138-1-AP)

    IP experiment of HeLa using 18138-1-AP

    IP result of anti-ABCD1 (IP:18138-1-AP, 4ug; Detection:18138-1-AP 1:700) with HeLa cells lysate 3000 ug.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using ABCD1 Polyclonal antibody (18138-1-AP)

    IHC staining of human ovary cancer using 18138-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 18138-1-AP (ABCD1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using ABCD1 Polyclonal antibody (18138-1-AP)

    IHC staining of human ovary cancer using 18138-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 18138-1-AP (ABCD1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using ABCD1 Polyclonal antibody (18138-1-AP)

    IF Staining of HeLa using 18138-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using ABCD1 antibody (18138-1-AP) at dilution of 1:1000 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

  • Flow cytometry (FC) experiment of HeLa cells using ABCD1 Polyclonal antibody (18138-1-AP)

    FC experiment of HeLa using 18138-1-AP

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human ABCD1 (18138-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive WB detected inHepG2 cells, HeLa cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

18138-1-AP targets ABCD1 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ABCD1 fusion protein Ag12757 相同性解析による交差性が予測される生物種
Full Name ATP-binding cassette, sub-family D (ALD), member 1
Calculated molecular weight 745 aa, 83 kDa
Observed molecular weight 75 kDa
GenBank accession numberBC025358
Gene symbol ABCD1
Gene ID (NCBI) 215
RRIDAB_2273333
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ABCD1 (also known as ALDP) is a member of the ATP-binding cassette (ABC) transporter superfamily which functions as transporter for a wide variety of substrates. It localizes to the peroxisomal membrane. The exact function is not clear so far. Various mutations of ABCD1 cause X-linked adrenoleukodystrophy (X-ALD), an inherited neurodegenerative disease affecting the nervous system white matter and adrenal cortex.

Protocols

Product Specific Protocols
WB protocol for ABCD1 antibody 18138-1-APDownload protocol
IHC protocol for ABCD1 antibody 18138-1-APDownload protocol
IF protocol for ABCD1 antibody 18138-1-APDownload protocol
IP protocol for ABCD1 antibody 18138-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

EMBO J

Insufficiency of ciliary cholesterol in hereditary Zellweger syndrome.

Authors - Tatsuo Miyamoto
  • KO Validated
View Article
humanWB

Signal Transduct Target Ther

Structural insights into substrate recognition and translocation of human peroxisomal ABC transporter ALDP

Authors - Chao Xiong
View Article