• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

  • Featured Product
  • KD/KO Validated

ABCD1 Polyclonal antibody, PBS Only

ABCD1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 18138-1-PBS

Synonyms

EC:3.1.2.-, ATP-binding cassette sub-family D member 1, ATP binding cassette sub family D member 1, ALD, ABCD 1

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using ABCD1 Polyclonal antibody (18138-1-AP)

    WB analysis using 18138-1-AP (same clone as 18138-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 18138-1-AP (ABCD1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18138-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using ABCD1 Polyclonal antibody (18138-1-AP)

    WB analysis of HeLa using 18138-1-AP (same clone as 18138-1-PBS)

    WB result of ABCD1 antibody (18138-1-AP, 1:600) with si-Control and si-ABCD1 transfected HeLa cells.. This data was developed using the same antibody clone with 18138-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using ABCD1 Polyclonal antibody (18138-1-AP)

    WB analysis of HepG2 using 18138-1-AP (same clone as 18138-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 18138-1-AP (ABCD1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18138-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using ABCD1 Polyclonal antibody (18138-1-AP)

    WB analysis of HeLa using 18138-1-AP (same clone as 18138-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 18138-1-AP (ABCD1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18138-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HeLa cells using ABCD1 Polyclonal antibody (18138-1-AP)

    IP experiment of HeLa using 18138-1-AP (same clone as 18138-1-PBS)

    IP result of anti-ABCD1 (IP:18138-1-AP, 4ug; Detection:18138-1-AP 1:700) with HeLa cells lysate 3000 ug. This data was developed using the same antibody clone with 18138-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using ABCD1 Polyclonal antibody (18138-1-AP)

    IHC staining of human ovary cancer using 18138-1-AP (same clone as 18138-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 18138-1-AP (ABCD1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18138-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using ABCD1 Polyclonal antibody (18138-1-AP)

    IHC staining of human ovary cancer using 18138-1-AP (same clone as 18138-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 18138-1-AP (ABCD1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18138-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using ABCD1 Polyclonal antibody (18138-1-AP)

    IF Staining of HeLa using 18138-1-AP (same clone as 18138-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using ABCD1 antibody (18138-1-AP) at dilution of 1:1000 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 18138-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using ABCD1 Polyclonal antibody (18138-1-AP)

    FC experiment of HeLa using 18138-1-AP (same clone as 18138-1-PBS)

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human ABCD1 (18138-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 18138-1-PBS in a different storage buffer formulation.

☆対象製品が30%オフ！組換えタンパク質&免染用試薬 >>> キャンペーン実施中！

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

18138-1-PBS targets ABCD1 in WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ABCD1 fusion protein Ag12757 相同性解析による交差性が予測される生物種
Full Name ATP-binding cassette, sub-family D (ALD), member 1
Calculated molecular weight 745 aa, 83 kDa
Observed molecular weight 75 kDa
GenBank accession numberBC025358
Gene symbol ABCD1
Gene ID (NCBI) 215
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

ABCD1 (also known as ALDP) is a member of the ATP-binding cassette (ABC) transporter superfamily which functions as transporter for a wide variety of substrates. It localizes to the peroxisomal membrane. The exact function is not clear so far. Various mutations of ABCD1 cause X-linked adrenoleukodystrophy (X-ALD), an inherited neurodegenerative disease affecting the nervous system white matter and adrenal cortex.