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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using ABCD1 Monoclonal antibody (60153-1-Ig)

    WB analysis of HeLa using 60153-1-Ig (same clone as 60153-1-PBS)

    WB result of ABCD1 antibody (60153-1-Ig, 1:1000) with si-Control and si-ABCD1 transfected HeLa cells. This data was developed using the same antibody clone with 60153-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using ABCD1 Monoclonal antibody (60153-1-Ig)

    WB analysis of HeLa using 60153-1-Ig (same clone as 60153-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 60153-1-Ig (ABCD1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60153-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using ABCD1 Monoclonal antibody (60153-1-Ig)

    IF Staining of HeLa using 60153-1-Ig (same clone as 60153-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using ABCD1 antibody (60153-1-Ig, Clone: 1H8E5 ) at dilution of 1:100 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).DAPI (blue). This data was developed using the same antibody clone with 60153-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

ABCD1 Monoclonal antibody, PBS Only

ABCD1 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 60153-1-PBS
Clone No.1H8E5

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

ABC42, ABCD1, Adrenoleukodystrophy protein, ALD, ALDP, AMN

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60153-1-PBS targets ABCD1 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen ABCD1 fusion protein Ag12757 相同性解析による交差性が予測される生物種
Full Name ATP-binding cassette, sub-family D (ALD), member 1
Calculated molecular weight 745 aa, 83 kDa
Observed molecular weight 75 kDa
GenBank accession numberBC025358
Gene Symbol ABCD1
Gene ID (NCBI) 215
RRIDAB_10640436
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP33897
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

ABCD1 (also known as ALDP) is a member of the ATP-binding cassette (ABC) transporter superfamily which functions as transporter for a wide variety of substrates. It localizes to the peroxisomal membrane. The exact function is not clear so far. Various mutations of ABCD1 cause X-linked adrenoleukodystrophy (X-ALD), an inherited neurodegenerative disease affecting the nervous system white matter and adrenal cortex.