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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse cerebellum tissue using ABCD2 Polyclonal antibody (18179-1-AP)

    WB analysis of mouse cerebellum using 18179-1-AP

    mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 18179-1-AP (ABCD2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of A431 cells using ABCD2 Polyclonal antibody (18179-1-AP)

    IP experiment of A431 using 18179-1-AP

    IP result of anti-ABCD2 (IP:18179-1-AP, 4ug; Detection:18179-1-AP 1:300) with A431 cells lysate 1200ug.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using ABCD2 Polyclonal antibody (18179-1-AP)

    IHC staining of human ovary cancer using 18179-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 18179-1-AP (ABCD2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using ABCD2 Polyclonal antibody (18179-1-AP)

    IHC staining of human ovary cancer using 18179-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 18179-1-AP (ABCD2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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ABCD2 Polyclonal antibody

ABCD2 Polyclonal Antibody for WB, IHC, IP, ELISA
Cat No. 18179-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, ELISA

Synonyms

EC:3.1.2.-, ATP-binding cassette sub-family D member 2, ALDRP, Adrenoleukodystrophy-related protein, Adrenoleukodystrophy-like 1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse cerebellum tissue
Positive IP detected inA431 cells
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

18179-1-AP targets ABCD2 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ABCD2 fusion protein Ag12872 相同性解析による交差性が予測される生物種
Full Name ATP-binding cassette, sub-family D (ALD), member 2
Calculated molecular weight 740 aa, 83 kDa
Observed molecular weight 75 kDa
GenBank accession numberBC104901
Gene Symbol ABCD2
Gene ID (NCBI) 225
RRIDAB_2288877
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UBJ2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for ABCD2 antibody 18179-1-APDownload protocol
IHC protocol for ABCD2 antibody 18179-1-APDownload protocol
IP protocol for ABCD2 antibody 18179-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Geroscience

Coordinated transcriptional upregulation of oxidative metabolism proteins in long-lived endocrine mutant mice

Authors - Ahmed M Elmansi
View Article