ABCD3 Monoclonal antibody

ABCD3 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG3

Reactivity

Human and More (1)

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1G7G5

Cat no : 66697-1-Ig

Synonyms

ABC43, ABCD3, PMP70, PXMP1



Tested Applications

Positive WB detected inHEK-293 cells, HepG2 cells, HT-29 cells, Caco-2 cells, THP-1 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:2000
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 2 publications below

Product Information

66697-1-Ig targets ABCD3 in WB, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Cited Reactivity human
Host / Isotype Mouse / IgG3
Class Monoclonal
Type Antibody
Immunogen ABCD3 fusion protein Ag24760 相同性解析による交差性が予測される生物種
Full Name ATP-binding cassette, sub-family D (ALD), member 3
Observed molecular weight 70 kDa
GenBank accession numberBC068509
Gene symbol ABCD3
Gene ID (NCBI) 5825
RRIDAB_2882050
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for ABCD3 antibody 66697-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle

Mol Cell

An Epstein-Barr virus protein interaction map reveals NLRP3 inflammasome evasion via MAVS UFMylation

Authors - Stephanie Pei Tung Yiu
humanWB

Life Sci Alliance

Non-canonical activation of the ER stress sensor ATF6 by Legionella pneumophila effectors

Authors - Nnejiuwa U Ibe
humanWB

Haematologica

Dimeric ferrochelatase bridges ABCB7 and ABCB10 homodimers in an architecturally defined molecular complex required for heme biosynthesis.

Authors - Nunziata Maio

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

PAULA (Verified Customer) (07-12-2023)

I tested this ABCD3 antibody at two dilutions on THP1 cells (20ug cell lysate) and I saw a nice band around 70 kDa, although some non-specific bands were also detected. Dilution 1:1000 showed similar results to 1:500 dilution.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000, 1:500
  • Cell Tissue Type: THP1 cell line
ABCD3 Antibody Western Blot validation (1:1000, 1:500 dilution) in THP1 cell line (Cat no:66697-1-Ig)