Validation Data Gallery
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Tested Applications
| Positive WB detected in | HeLa cells, HepG2 cells, Jurkat cells, mouse liver tissue, mouse skeletal muscle tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
15935-1-AP targets ABCD4 in WB, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | ABCD4 fusion protein Ag8783 相同性解析による交差性が予測される生物種 |
| Full Name | ATP-binding cassette, sub-family D (ALD), member 4 |
| Calculated molecular weight | 606 aa, 69 kDa |
| Observed molecular weight | 70 kDa |
| GenBank accession number | BC012815 |
| Gene Symbol | ABCD4 |
| Gene ID (NCBI) | 5826 |
| RRID | AB_3085475 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O14678 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
ABCD4, also known as P70R, PMP69, and PXMP1L, belongs to the ATP-binding cassette (ABC) transporter superfamily. ABCD4 Lysosomal membrane protein that transports cobalamin (Vitamin B12) from the lysosomal lumen to the cytosol in an ATP-dependent manner (PMID: 22922874, 33845046, 28572511). ABCD4 is involved in peroxisomal import of fatty acids and/or fatty acyl-CoAs in the organelle. Mutations in ABCD4 cause a new inborn error of vitamin B12 metabolism, which is related to methylmalonic aciduria and homocystinuria type cblJ (MAHCJ).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for ABCD4 antibody 15935-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |