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  • KD/KO Validated

ABCG1 Polyclonal antibody

ABCG1 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, ELISA

Conjugate

Unconjugated

Cat no : 13578-1-AP

Synonyms

ABC8, ABCG1, White protein homolog, WHITE1, WHT1


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Tested Applications

Positive WB detected inHUVEC cells, RAW 264.7 cells, HeLa cells, HepG2 cells, mouse kidney tissue, rat kidney tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inmouse placenta tissue, human tonsillitis tissue, human cervical cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13578-1-AP targets ABCG1 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ABCG1 fusion protein Ag4479 相同性解析による交差性が予測される生物種
Full Name ATP-binding cassette, sub-family G (WHITE), member 1
Calculated molecular weight 678 aa, 76 kDa
Observed molecular weight 75 kDa
GenBank accession numberBC029158
Gene symbol ABCG1
Gene ID (NCBI) 9619
RRIDAB_2220174
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ABCG1 is a cholesterol and phospholipid transporter expressed in lung, brain, spleen, and macrophages. In liver, ABCG1 is expressed mainly in Kupffer cells. The monomer of ABCG1 is around 60-65 kDa, while multiple forms of ABCG1 can be observed on SDS-PAGE, including 130 kDa (dimer), 90-95 kDa, 110 kDa. It was not clear whether the glycosylation or splicing accounts for the differences in mass. (PMID: 16702602)

Protocols

Product Specific Protocols
WB protocol for ABCG1 antibody 13578-1-APDownload protocol
IHC protocol for ABCG1 antibody 13578-1-APDownload protocol
IP protocol for ABCG1 antibody 13578-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cancer Res

PRMT1 sustains de novo fatty acid synthesis by methylating PHGDH to drive chemoresistance in triple-negative breast cancer

Authors - Takehiro Yamamoto
mouseWB

Autophagy

Metabolic effects of RUBCN/Rubicon deficiency in kidney proximal tubular epithelial cells.

Authors - Jun Matsuda
humanWB

Br J Pharmacol

Functional interplay between LXR and AMPKα inhibits atherosclerosis in apoE deficient mice - A new anti-atherogenic strategy.

Authors - Chuanrui Ma
humanIF

J Pharm Anal

Targeted delivery of rosuvastatin enhances treatment of hyperhomocysteinemia-induced atherosclerosis using macrophage membrane-coated nanoparticles

Authors - Dayue Liu
mouseIHC

Phytomedicine

Rice bran active peptide (RBAP) inhibited macrophage differentiation to foam cell and atherosclerosis in mice via regulating cholesterol efflux

Authors - Jianfei Mu
mouseWB,IF

Phytomedicine

MaiJiTong granule attenuates atherosclerosis by reducing ferroptosis via activating STAT6-mediated inhibition of DMT1 and SOCS1/p53 pathways in LDLR-/- mice

Authors - Jia Shi

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Shiford (Verified Customer) (09-24-2023)

We sorted cancer stem cell subpopulations from multiple cell lines by flow cytometry and used a cluster of antibodies related to stem cell characteristics to detect. ABCG1 is one of the possible stem cell markers, with clear bands.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1500
  • Cell Tissue Type: Cancer cell lines from various tissues
ABCG1 Antibody Western Blot validation (1:1500 dilution) in Cancer cell lines from various tissues (Cat no:13578-1-AP)