Validation Data Gallery
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Tested Applications
| Positive WB detected in | HL-60 cells, PC-12 cells, k-562 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 2 publications below |
Product Information
24453-1-AP targets ABCG8 in WB, ELISA applications and shows reactivity with human, rat samples.
| Tested Reactivity | human, rat |
| Cited Reactivity | mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | ABCG8 fusion protein Ag19879 相同性解析による交差性が予測される生物種 |
| Full Name | ATP-binding cassette, sub-family G (WHITE), member 8 |
| Calculated molecular weight | 673 aa, 76 kDa |
| Observed molecular weight | 70 kDa |
| GenBank accession number | BC113657 |
| Gene Symbol | ABCG8 |
| Gene ID (NCBI) | 64241 |
| RRID | AB_3085735 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9H221 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
ABCG8(ATP-binding cassette sub-family G member 8) and ABCG5(ATP-binding cassette protein G5) are members of the ABC transporter family and are half-type ABC transporters, which consist of six transmembrane helices and one nucleotide-binding domain. ABCG5 and ABCG8 form a heterodimer (ABCG5/ABCG8). Mutations in either ABCG5 or ABCG8 cause a genetic disorder, sitosterolemia. Patients with sitosterolemia show high plant sterol levels and develop hypercholesterolemia and atherosclerosis at a young age.(PMID: 36839356)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for ABCG8 antibody 24453-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Naunyn Schmiedebergs Arch Pharmacol Molecular mechanism of geniposide against ANIT-induced intrahepatic cholestasis by integrative analysis of transcriptomics and metabolomics | ||
Drug Des Devel Ther Baolier Capsule's Secret Weapon: Piperine Boosts Cholesterol Excretion to Combat Atherosclerosis |