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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ABHD11 Polyclonal antibody (25355-1-AP)

    WB analysis using 25355-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25355-1-AP (ABHD11 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of PC-3 cells using ABHD11 Polyclonal antibody (25355-1-AP)

    IF Staining of PC-3 using 25355-1-AP

    Immunofluorescent analysis of (4% PFA) fixed PC-3 cells using ABHD11 antibody (25355-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

ABHD11 Polyclonal antibody

ABHD11 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 25355-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

sn-1-specific diacylglycerol lipase ABHD11, PP1226, EC:3.1.1.116, Alpha/beta hydrolase domain-containing protein 11, Abhydrolase domain-containing protein 11

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inMCF-7 cells, PC-3 cells, human placenta tissue
Positive IF/ICC detected inPC-3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

25355-1-AP targets ABHD11 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ABHD11 fusion protein Ag18318 相同性解析による交差性が予測される生物種
Full Name abhydrolase domain containing 11
Calculated molecular weight 315 aa, 35 kDa
Observed molecular weight30 kDa
GenBank accession numberBC067750
Gene Symbol ABHD11
Gene ID (NCBI) 83451
RRIDAB_3669471
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8NFV4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Abhydrolase domain-containing protein 11 (ABHD11) is a serine hydrolase enzyme, localized in mitochondria, that can synthesize the endocannabinoid 2-arachidonoyl glycerol (2AG) in vitro.

Protocols

Product Specific Protocols
WB protocol for ABHD11 antibody 25355-1-APDownload protocol
IF protocol for ABHD11 antibody 25355-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols