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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse kidney tissue using ABHD14A Polyclonal antibody (19419-1-AP)

    WB analysis of mouse kidney using 19419-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 19419-1-AP (ABHD14A antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse kidney tissue using ABHD14A Polyclonal antibody (19419-1-AP)

    WB analysis of mouse kidney using 19419-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 19419-1-AP (ABHD14A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse kidney tissue using ABHD14A Polyclonal antibody (19419-1-AP)

    IP experiment of mouse kidney using 19419-1-AP

    IP result of anti-ABHD14A (IP:19419-1-AP, 4ug; Detection:19419-1-AP 1:300) with mouse kidney tissue lysate 4000ug.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using ABHD14A Polyclonal antibody (19419-1-AP)

    IF Staining of HeLa using 19419-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 19419-1-AP (ABHD14A antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using ABHD14A Polyclonal antibody (19419-1-AP)

    IF Staining of HeLa using 19419-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using ABHD14A antibody (19419-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4).

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ABHD14A Polyclonal antibody

ABHD14A Polyclonal Antibody for WB, IF/ICC, IP, ELISA
Cat No. 19419-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, IP, ELISA

Synonyms

Protein ABHD14A, EC:3.-.-.-, DORZ1, Alpha/beta hydrolase domain-containing protein 14A, Abhydrolase domain-containing protein 14A

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse kidney tissue
Positive IP detected inmouse kidney tissue
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

19419-1-AP targets ABHD14A in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ABHD14A fusion protein Ag13744 相同性解析による交差性が予測される生物種
Full Name abhydrolase domain containing 14A
Calculated molecular weight 271 aa, 30 kDa
Observed molecular weight 30-32 kDa
GenBank accession numberBC002571
Gene Symbol ABHD14A
Gene ID (NCBI) 25864
RRIDAB_10642140
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BUJ0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for ABHD14A antibody 19419-1-APDownload protocol
IF protocol for ABHD14A antibody 19419-1-APDownload protocol
IP protocol for ABHD14A antibody 19419-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols