Validation Data Gallery
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Tested Applications
| Positive WB detected in | mouse lung tissue, Transfected HEK-293 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 2 publications below |
Product Information
22526-1-AP targets ABHD15 in WB, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | ABHD15 fusion protein Ag18334 相同性解析による交差性が予測される生物種 |
| Full Name | abhydrolase domain containing 15 |
| Calculated molecular weight | 468 aa, 52 kDa |
| Observed molecular weight | 52 kDa |
| GenBank accession number | BC012476 |
| Gene Symbol | ABHD15 |
| Gene ID (NCBI) | 116236 |
| RRID | AB_2879117 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q6UXT9 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
ABHD15 belongs to the α/β-hydrolase superfamily which consists of various lipases, esterases, and proteases that share an α/β fold as a common structural feature. ABHD15 is highly expressed in adipose tissues and is required for the anti-lipolytic action of INS in white adipose tissue (WAT). ABHD15 expression is decreased in humans with obesity and diabetes compared to humans with obesity and normal glucose tolerance, identifying ABHD15 as a potential therapeutic target to mitigate INS resistance (PMID: 29768196).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for ABHD15 antibody 22526-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Mol Metab ABHD15 regulates adipose tissue lipolysis and hepatic lipid accumulation.
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