Validation Data Gallery
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Tested Applications
| Positive WB detected in | HepG2 cells, HEK-293 cells |
| Positive IHC detected in | human stomach tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HEK-293 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 6 publications below |
| IHC | See 1 publications below |
| IF | See 4 publications below |
Product Information
14039-1-AP targets ABHD2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | ABHD2 fusion protein Ag5108 相同性解析による交差性が予測される生物種 |
| Full Name | abhydrolase domain containing 2 |
| Calculated molecular weight | 48 kDa |
| Observed molecular weight | 48 kDa |
| GenBank accession number | BC019248 |
| Gene Symbol | ABHD2 |
| Gene ID (NCBI) | 11057 |
| RRID | AB_2242089 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P08910 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for ABHD2 antibody 14039-1-AP | Download protocol |
| IHC protocol for ABHD2 antibody 14039-1-AP | Download protocol |
| IF protocol for ABHD2 antibody 14039-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Science Unconventional endocannabinoid signaling governs sperm activation via the sex hormone progesterone. | ||
Front Cell Dev Biol Alpha/Beta Hydrolase Domain-Containing Protein 2 Regulates the Rhythm of Follicular Maturation and Estrous Stages of the Female Reproductive Cycle.
| ||
J Gen Physiol The epithelial potassium channel Kir7.1 is stimulated by progesterone.
| ||
Biochem Biophys Res Commun Regulation of calcium release from the endoplasmic reticulum by the serine hydrolase ABHD2. | ||
Int J Mol Sci Retinoic Acid Induces Differentiation of Mouse F9 Embryonic Carcinoma Cell by Modulating the miR-485 Targeting of Abhd2. | ||
Cancer Med Vaspin accelerates the proliferation, invasion and metastasis of Triple-Negative breast cancer through MiR-33a-5p/ABHD2 |