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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ABHD5 Monoclonal antibody, PBS Only

ABHD5 Monoclonal Antibody for WB, IF, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

WB, IF, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2B12C2

Cat no : 67779-1-PBS

Synonyms

ABHD5, CDS, CGI58, IECN2, NCIE2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ABHD5 Monoclonal antibody (67779-1-Ig)

    WB analysis using 67779-1-Ig (same clone as 67779-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67779-1-Ig (ABHD5 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67779-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using ABHD5 Monoclonal antibody (67779-1-Ig)

    IF Staining of HepG2 using 67779-1-Ig (same clone as 67779-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using ABHD5 antibody (67779-1-Ig, Clone: 2B12C2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67779-1-PBS in a different storage buffer formulation.

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67779-1-PBS targets ABHD5 in WB, IF, Indirect ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen ABHD5 fusion protein Ag30256 相同性解析による交差性が予測される生物種
Full Name abhydrolase domain containing 5
Calculated molecular weight 349 aa, 39 kDa
Observed molecular weight39 kDa
GenBank accession numberBC021958
Gene symbol ABHD5
Gene ID (NCBI) 51099
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

ABHD5, also named as NCIE2 and CGI-58, belongs to the peptidase S33 family. ABHD4/ABHD5 subfamily. It is a Lysophosphatidic acid acyltransferase which functions in phosphatidic acid biosynthesis. ABHD5 may regulate the cellular storage of triacylglycerol through activation of the phospholipase PNPLA2. It is involved in keratinocyte differentiation. ABHD5 is an evolutionarily conserved protein that acts as a potent activator of Atgl. Abhd5 is expressed in several tissues that lack Plin , raising the possibility that this co-activator might interact with additional PAT proteins. Abhd5 and Mldp are highly colocalized on individual lipid droplets.(PMID:19064991) Defects in ABHD5 are the cause of Chanarin-Dorfman syndrome (CDS).