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KD/KO Validated

ABI1 Polyclonal antibody

Name: ABI1 Polyclonal antibody
Brand: Proteintech
SKU: 27387-1-AP

ABI1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, CoIP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 27387-1-AP

Synonyms

Abelson interactor 1, ABI 1, ABI1, Abl binding protein 4, abl interactor 1, AblBP4, E3B1, Eps8 binding protein, Nap1 binding protein, NAP1BP, SSH3BP, SSH3BP1

Validation Data Gallery

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AllWBIHC

WB analysis using 27387-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 27387-1-AP (ABI1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB analysis using 27387-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 27387-1-AP (ABI1 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293T using 27387-1-AP
HEK-293T cells were subjected to SDS PAGE followed by western blot with 27387-1-AP (ABI1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of C2C12 using 27387-1-AP
C2C12 cell were subjected to SDS PAGE followed by western blot with 27387-1-AP (ABI1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of MCF-7 using 27387-1-AP
MCF-7 cells were subjected to SDS PAGE followed by western blot with 27387-1-AP (ABI1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
IHC staining of mouse skin using 27387-1-AP
Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 27387-1-AP (ABI1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse skin using 27387-1-AP
Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 27387-1-AP (ABI1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 27387-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 27387-1-AP (ABI1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 27387-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 27387-1-AP (ABI1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Cat No. 27387-1-AP

Size/Concentration

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	Neuro-2a cells, C2C12 cells, multi cells, HEK-293T cells, HepG2 cells, HEK 293 cells, Jurkat cells, mouse cerebellum tissue, rat cerebellum tissue
Positive IHC detected in	mouse skin tissue, mouse brain tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:5000-1:50000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 2 publications below
IF	See 1 publications below
CoIP	See 1 publications below

Product Information

27387-1-AP targets ABI1 in WB, IF, IHC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	ABI1 fusion protein Ag26537 相同性解析による交差性が予測される生物種
Full Name	abl-interactor 1
Calculated molecular weight	507 aa, 55 kDa
Observed molecular weight	55-60 kDa
GenBank accession number	BC024254
Gene symbol	ABI1
Gene ID (NCBI)	10006
RRID	AB_2880860
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for ABI1 antibody 27387-1-AP	Download protocol
IHC protocol for ABI1 antibody 27387-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	J Psychiatr Res Circulating miR-30e-3p induces disruption of neurite development in SH-SY5Y cells by targeting ABI1, a novel biomarker for schizophrenia Authors - Mengdi Jin KD Validated View Article
human	WB,IF,CoIP	Transl Oncol CBLC promotes the development of colorectal cancer by promoting ABI1 degradation to activate the ERK signaling pathway Authors - Zhan Li View Article

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Species Reactivity

Species Reactivity

Conjugate

Conjugate

ABI1 Polyclonal antibody

ABI1 Polyclonal Antibody for WB, IHC, ELISA

Validation Data Gallery

WB analysis using 27387-1-AP

WB analysis using 27387-1-AP

WB analysis of HEK-293T using 27387-1-AP

WB analysis of C2C12 using 27387-1-AP

WB analysis of MCF-7 using 27387-1-AP

IHC staining of mouse skin using 27387-1-AP

IHC staining of mouse skin using 27387-1-AP

IHC staining of mouse brain using 27387-1-AP