ABI2 Polyclonal antibody

Name: ABI2 Polyclonal antibody
Brand: Proteintech
SKU: 14890-1-AP

ABI2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, CoIP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 14890-1-AP

Synonyms

Abelson interactor 2, ABI 2, ABI2, ABI2B, Abl binding protein 3, abl interactor 2, AblBP3, AIP 1, Arg binding protein 1, ArgBP1, argBPIA, argBPIB, SSH3BP2

Validation Data Gallery

Filter:

AllWBIHC

WB analysis using 14890-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 14890-1-AP (ABI2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB analysis of mouse heart using 14890-1-AP
mouse heart tissue were subjected to SDS PAGE followed by western blot with 14890-1-AP (ABI2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse uterus using 14890-1-AP
mouse uterus tissue were subjected to SDS PAGE followed by western blot with 14890-1-AP (ABI2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 14890-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 14890-1-AP (ABI2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse testis using 14890-1-AP
mouse testis tissue were subjected to SDS PAGE followed by western blot with 14890-1-AP (ABI2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 14890-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 14890-1-AP (ABI2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
IHC staining of human brain using 14890-1-AP
Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 14890-1-AP (ABI2 Antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human brain using 14890-1-AP
Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 14890-1-AP (ABI2 Antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human testis using 14890-1-AP
Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 14890-1-AP (ABI2 Antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human testis using 14890-1-AP
Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 14890-1-AP (ABI2 Antibody) at dilution of 1:50 (under 40x lens).

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Cat No. 14890-1-AP

Size/Concentration

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	HEK-293 cells, mouse heart tissue, mouse uterus tissue, mouse testis tissue, mouse brain tissue, HeLa cells, K-562 cells, SH-SY5Y cells
Positive IHC detected in	human brain tissue, human testis tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:6000
Immunohistochemistry (IHC)	IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 3 publications below
CoIP	See 1 publications below

Product Information

14890-1-AP targets ABI2 in WB, IHC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	ABI2 fusion protein Ag6679 相同性解析による交差性が予測される生物種
Full Name	abl interactor 2
Calculated molecular weight	56 kDa
Observed molecular weight	52-56 kDa
GenBank accession number	BC001439
Gene symbol	ABI2
Gene ID (NCBI)	10152
RRID	AB_2288997
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for ABI2 antibody 14890-1-AP	Download protocol
IHC protocol for ABI2 antibody 14890-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB,CoIP	Nat Commun GRAF1 integrates PINK1-Parkin signaling and actin dynamics to mediate cardiac mitochondrial homeostasis Authors - Qiang Zhu View Article
mouse,human	WB	Nat Commun hnRNP A1 dysfunction alters RNA splicing and drives neurodegeneration in multiple sclerosis (MS) Authors - Hannah E Salapa View Article
human	WB	Nat Cell Biol Actin cytoskeleton vulnerability to disulfide stress mediates disulfidptosis Authors - Xiaoguang Liu View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ABI2 Polyclonal antibody

ABI2 Polyclonal Antibody for WB, IHC, ELISA

Validation Data Gallery

WB analysis using 14890-1-AP

WB analysis of mouse heart using 14890-1-AP

WB analysis of mouse uterus using 14890-1-AP

WB analysis of HEK-293 using 14890-1-AP

WB analysis of mouse testis using 14890-1-AP

WB analysis of mouse brain using 14890-1-AP

IHC staining of human brain using 14890-1-AP

IHC staining of human brain using 14890-1-AP

IHC staining of human testis using 14890-1-AP