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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ABLIM3 Polyclonal antibody

ABLIM3 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 27981-1-AP

Synonyms

abLIM 3, ABLIM3, Actin binding LIM protein 3, HMFN1661, KIAA0843

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ABLIM3 Polyclonal antibody (27981-1-AP)

    WB analysis using 27981-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27981-1-AP (ABLIM3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using ABLIM3 Polyclonal antibody (27981-1-AP)

    WB analysis of mouse brain using 27981-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 27981-1-AP (ABLIM3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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Tested Applications

Positive WB detected inNIH/3T3 cells, mouse brain tissue, HepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

27981-1-AP targets ABLIM3 in WB, ELISA applications and shows reactivity with Human, Mouse samples.

Tested Reactivity Human, Mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ABLIM3 fusion protein Ag27578 相同性解析による交差性が予測される生物種
Full Name actin binding LIM protein family, member 3
Calculated molecular weight 78 kDa
Observed molecular weight 78 kDa
GenBank accession numberBC001665
Gene symbol ABLIM3
Gene ID (NCBI) 22885
RRIDAB_2881029
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for ABLIM3 antibody 27981-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
WB

Mol Biol Cell

Spatial proximity of proteins surrounding zyxin under force-bearing conditions.

Authors - Joleen S Cheah
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Joleen (Verified Customer) (05-29-2019)

It works on western blot with a prominent band and little background.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1k
  • Cell Tissue Type: MDCK GII
ABLIM3 Antibody Western Blot, validation (1:1k dilution) in MDCK GII (Cat no:27981-1-AP)