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ACAA2 Polyclonal antibody

ACAA2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

Cat no : 11111-1-AP

Synonyms

Beta ketothiolase, Acyl-CoA hydrolase, mitochondrial, Acetyl-CoA acyltransferase, Acetyl-CoA acetyltransferase, Acetyl CoA acyltransferase

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ACAA2 Polyclonal antibody (11111-1-AP)

    WB analysis using 11111-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11111-1-AP (ACAA2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using ACAA2 Polyclonal antibody (11111-1-AP)

    WB analysis of HEK-293 using 11111-1-AP

    WB result of ACAA2 antibody (11111-1-AP; 1:300; incubated at room temperature for 1.5 hours) with sh-Control and sh-ACAA2 transfected HEK-293 cells.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using ACAA2 Polyclonal antibody (11111-1-AP)

    IHC staining of human lung cancer using 11111-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11111-1-AP (ACAA2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon tissue using ACAA2 Polyclonal antibody (11111-1-AP)

    IHC staining of human colon using 11111-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 11111-1-AP (ACAA2 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse liver tissue using ACAA2 Polyclonal antibody (11111-1-AP)

    IHC staining of mouse liver using 11111-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 11111-1-AP (ACAA2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver tissue using ACAA2 Polyclonal antibody (11111-1-AP)

    IHC staining of human liver using 11111-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 11111-1-AP (ACAA2 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using ACAA2 Polyclonal antibody (11111-1-AP)

    IHC staining of human liver using 11111-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 11111-1-AP (ACAA2 antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

Positive WB detected inHEK-293 cells, HepG2 cells, NIH/3T3 cells
Positive IHC detected inhuman lung cancer tissue, human colon tissue, human liver tissue, mouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11111-1-AP targets ACAA2 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, sheep
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ACAA2 fusion protein Ag1609 相同性解析による交差性が予測される生物種
Full Name acetyl-Coenzyme A acyltransferase 2
Calculated molecular weight 42 kDa
Observed molecular weight 42 kDa
GenBank accession numberBC001918
Gene symbol ACAA2
Gene ID (NCBI) 10449
RRIDAB_2219389
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for ACAA2 antibody 11111-1-APDownload protocol
IHC protocol for ACAA2 antibody 11111-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Oncotarget

Genomic binding and regulation of gene expression by the thyroid carcinoma-associated PAX8-PPARG fusion protein.

Authors - Yanxiao Zhang
View Article
ratWB

EBioMedicine

PPARγ maintains the metabolic heterogeneity and homeostasis of renal tubules.

Authors - Zhongshi Lyu
View Article
ratWB,IHC

PLoS One

Pressure load: the main factor for altered gene expression in right ventricular hypertrophy in chronic hypoxic rats.

Authors - Baandrup Jonas D JD
View Article
sheepWB

Front Genet

Identification of miRNA in Sheep Intramuscular Fat and the Role of miR-193a-5p in Proliferation and Differentiation of 3T3-L1.

Authors - Fuhui Han
View Article
ratWB

Gerontology

Dietary Restriction Increases Protein Acetylation in the Livers of Aged Rats.

Authors - Nakamura Akihiro A
View Article
humanWB

J Biol Chem

CLPX regulates mitochondrial fatty acid β-oxidation in liver cells

Authors - Ko Suzuki
View Article