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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ACAD10 Polyclonal antibody

ACAD10 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 17161-1-AP

Synonyms

EC:1.3.99.-, Acyl-CoA dehydrogenase family member 10, ACAD-10, ACAD 10

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse spleen tissue using ACAD10 Polyclonal antibody (17161-1-AP)

    WB analysis of mouse spleen using 17161-1-AP

    mouse spleen tissue were subjected to SDS PAGE followed by western blot with 17161-1-AP (ACAD10 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human pancreas tissue using ACAD10 Polyclonal antibody (17161-1-AP)

    IHC staining of human pancreas using 17161-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas using 17161-1-AP (ACAD10 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human pancreas tissue using ACAD10 Polyclonal antibody (17161-1-AP)

    IHC staining of human pancreas using 17161-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas using 17161-1-AP (ACAD10 antibody) at dilution of 1:100 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using ACAD10 Polyclonal antibody (17161-1-AP)

    IF Staining of HeLa using 17161-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using ACAD10 antibody (17161-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

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Tested Applications

Positive WB detected inmouse spleen tissue
Positive IHC detected inhuman pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17161-1-AP targets ACAD10 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ACAD10 fusion protein Ag10510 相同性解析による交差性が予測される生物種
Full Name acyl-Coenzyme A dehydrogenase family, member 10
Calculated molecular weight 26 kDa, 119 kDa
Observed molecular weight 119 kDa
GenBank accession numberBC015056
Gene symbol ACAD10
Gene ID (NCBI) 80724
RRIDAB_2219552
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ACAD10(Acyl-CoA dehydrogenase family member 10) belongs to the acyl-CoA dehydrogenase family. ACAD10 has significant activity towards the branched-chain substrates R and S, 2 methyl-C15-CoA and is highly expressed in fetal but not adult brain. This pattern of expression is similar to that of LCAD, another ACAD previously shown to be involved in long branched chain fatty acid metabolism(PMID:21237683). It has 4 isoforms produced by alternative splicing.

Protocols

Product Specific Protocols
WB protocol for ACAD10 antibody 17161-1-APDownload protocol
IHC protocol for ACAD10 antibody 17161-1-APDownload protocol
IF protocol for ACAD10 antibody 17161-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseWB

Cell Mol Life Sci

ACAD10 and ACAD11 allow entry of 4-hydroxy fatty acids into β-oxidation

Authors - Stéphanie Paquay
  • KO Validated
View Article