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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse pancreas tissue using ACAD8 Polyclonal antibody (16742-1-AP)

    WB analysis of mouse pancreas using 16742-1-AP

    mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 16742-1-AP (ACAD8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse lung tissue using ACAD8 Polyclonal antibody (16742-1-AP)

    WB analysis of mouse lung using 16742-1-AP

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 16742-1-AP (ACAD8 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse lung tissue using ACAD8 Polyclonal antibody (16742-1-AP)

    WB analysis of mouse lung using 16742-1-AP

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 16742-1-AP (ACAD8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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ACAD8 Polyclonal antibody

ACAD8 Polyclonal Antibody for WB, ELISA
Cat No. 16742-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

ACAD 8, ACAD8, ARC42, IBD

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse pancreas tissue, mouse lung tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16742-1-AP targets ACAD8 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ACAD8 fusion protein Ag9967 相同性解析による交差性が予測される生物種
Full Name acyl-Coenzyme A dehydrogenase family, member 8
Calculated molecular weight 45 kDa
Observed molecular weight 45 kDa
GenBank accession numberBC001964
Gene Symbol ACAD8
Gene ID (NCBI) 27034
RRIDAB_2219559
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UKU7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The acyl-CoA dehydrogenases (ACAD) (EC 1.3.99) are a family of nuclear-encoded mitochondrial enzymes involved in the metabolism of fatty acids and branched chain amino acids (PMID: 13295225). The eighth member of this family, ACAD8, catalyzes the conversion of isobutyryl-CoA to methacrylyl-CoA in valine catabolism (PMID: 12359132). ACAD8, also named as acyl-Coenzyme A dehydrogenase family, member 8, has 3 isoform which molecular weight is 45, 31 and 38 kDa.

Protocols

Product Specific Protocols
WB protocol for ACAD8 antibody 16742-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols