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ACAD9 Polyclonal antibody
ACAD9 Polyclonal Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
WB, IHC, ELISA
Conjugate
Unconjugated
Cat no : 15770-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HEK-293T cells, MCF-7 cells |
Positive IHC detected in | human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:1000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
KD/KO | See 1 publications below |
WB | See 4 publications below |
Product Information
15770-1-AP targets ACAD9 in WB, IHC, ELISA applications and shows reactivity with human samples.
Tested Reactivity | human |
Cited Reactivity | human |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | ACAD9 fusion protein Ag8414 相同性解析による交差性が予測される生物種 |
Full Name | acyl-Coenzyme A dehydrogenase family, member 9 |
Calculated molecular weight | 69 kDa |
Observed molecular weight | 65 kDa |
GenBank accession number | BC007970 |
Gene symbol | ACAD9 |
Gene ID (NCBI) | 28976 |
RRID | AB_2241997 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Acyl-CoA dehydrogenases (ACADs) are a family of mitochondrial enzymes catalyzing the initial rate-limiting step in the β-oxidation of fatty acyl-CoA. ACAD9 belongs to the group of ACADs. The deduced 621-amino acid protein has a calculated molecular mass of 68.8 kD. It has an N-terminal leader sequence, 2 conserved motifs shared by all ACAD family members, and a potential N-glycosylation site(PMID:12359260). Defects in ACAD9 are a cause of acyl-CoA dehydrogenase family member type 9 deficiency (ACAD9 deficiency).
Protocols
Product Specific Protocols | |
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WB protocol for ACAD9 antibody 15770-1-AP | Download protocol |
IHC protocol for ACAD9 antibody 15770-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
EMBO Rep MITRAC15/COA1 promotes mitochondrial translation in a ND2 ribosome-nascent chain complex. | ||
Sci Rep Integrative analyses of translatome and transcriptome reveal important translational controls in brown and white adipose regulated by microRNAs. | ||
Cell Rep A membrane arm of mitochondrial complex I sufficient to promote respirasome formation.
| ||
Front Neurosci Ultrasound stimulation of the vagal nerve improves acute septic encephalopathy in mice |