ACADL Polyclonal antibody

Name: ACADL Polyclonal antibody
Brand: Proteintech
SKU: 17442-1-AP

ACADL Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 17442-1-AP

Synonyms

ACAD4, ACADL, LCAD

Validation Data Gallery

Filter:

AllWBIHC

WB analysis using 17442-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 17442-1-AP (ACADL antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 17442-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 17442-1-AP (ACADL antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 17442-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 17442-1-AP (ACADL antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse kidney using 17442-1-AP
mouse kidney tissue were subjected to SDS PAGE followed by western blot with 17442-1-AP (ACADL antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse kidney using 17442-1-AP
mouse kidney tissue were subjected to SDS PAGE followed by western blot with 17442-1-AP (ACADL antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of NIH/3T3 using 17442-1-AP
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 17442-1-AP (ACADL antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 17442-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 17442-1-AP (ACADL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 17442-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 17442-1-AP (ACADL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse kidney using 17442-1-AP
mouse kidney tissue were subjected to SDS PAGE followed by western blot with 17442-1-AP (ACADL antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 17442-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 17442-1-AP (ACADL antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 17442-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 17442-1-AP (ACADL antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
WB analysis of rat kidney using 17442-1-AP
rat kidney tissue were subjected to SDS PAGE followed by western blot with 17442-1-AP (ACADL antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
IHC staining of human liver cancer using 17442-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17442-1-AP (ACADL antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 17442-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17442-1-AP (ACADL antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver using 17442-1-AP
Immunohistochemical analysis of paraffin-embedded human liver slide using 17442-1-AP (ACADL Antibody) at dilution of 1:50.
IHC staining of human liver using 17442-1-AP
Immunohistochemical analysis of paraffin-embedded human liver slide using 17442-1-AP (ACADL Antibody) at dilution of 1:50.

-/ -

Cat No. 17442-1-AP

Size/Concentration

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	mouse kidney tissue, HepG2 cells, rat kidney tissue, HeLa cells, HEK-293 cells, NIH/3T3 cells
Positive IHC detected in	human liver cancer tissue, human liver tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:6000
Immunohistochemistry (IHC)	IHC : 1:100-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 5 publications below

Product Information

17442-1-AP targets ACADL in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, rat, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	ACADL fusion protein Ag11530 相同性解析による交差性が予測される生物種
Full Name	acyl-Coenzyme A dehydrogenase, long chain
Calculated molecular weight	430 aa, 48 kDa
Observed molecular weight	45-48 kDa
GenBank accession number	BC039063
Gene symbol	ACADL
Gene ID (NCBI)	33
RRID	AB_2219662
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

ACADL(Long-chain specific acyl-CoA dehydrogenase, mitochondrial) is also named as LCAD and belongs to the acyl-CoA dehydrogenase family.It catalyzes the first reaction of the mitochondrial β-oxidation of fatty acids and is synthesized in the cytosol as a precursor that is larger than its mature from. The size difference between the precursor and mature rat LCAD has been estimated to be 3 kDa on the basis of SDS-PAGE(PMID:1774065).Defects in ACADL are a cause of acyl-CoA dehydrogenase very long-chain deficiency (ACADVLD).

Protocols

Product Specific Protocols
WB protocol for ACADL antibody 17442-1-AP	Download protocol
IHC protocol for ACADL antibody 17442-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Nat Commun Enoyl-CoA hydratase-1 regulates mTOR signaling and apoptosis by sensing nutrients. Authors - Ya-Kun Zhang View Article
rat	WB	Front Pharmacol Lipidomics reveals the potential mechanism of honokiol against adenine-induced chronic kidney disease Authors - Xinhui Liu View Article
mouse	WB	Front Pharmacol The serum acylcarnitines profile in epileptic children treated with valproic acid and the protective roles of peroxisome proliferator-activated receptor a activation in valproic acid-induced liver injury Authors - Yiyi Ma View Article
human,mouse	WB	Diabetologia Chaperonin counteracts diet-induced non-alcoholic fatty liver disease by aiding sirtuin 3 in the control of fatty acid oxidation Authors - Shao-Wen Weng View Article
human	WB	Cancer Metab A HIF-1α inhibitor combined with palmitic acid and L-carnitine treatment can prevent the fat metabolic reprogramming under hypoxia and induce apoptosis in hepatocellular carcinoma cells Authors - Shohei Matsufuji View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ACADL Polyclonal antibody

ACADL Polyclonal Antibody for WB, IHC, ELISA

Validation Data Gallery

WB analysis using 17442-1-AP

WB analysis of HeLa using 17442-1-AP

WB analysis of HeLa using 17442-1-AP

WB analysis of mouse kidney using 17442-1-AP

WB analysis of mouse kidney using 17442-1-AP

WB analysis of NIH/3T3 using 17442-1-AP

WB analysis of HEK-293 using 17442-1-AP

WB analysis of HEK-293 using 17442-1-AP

WB analysis of mouse kidney using 17442-1-AP

WB analysis of HeLa using 17442-1-AP

WB analysis of HepG2 using 17442-1-AP

WB analysis of rat kidney using 17442-1-AP

IHC staining of human liver cancer using 17442-1-AP

IHC staining of human liver cancer using 17442-1-AP

IHC staining of human liver using 17442-1-AP

IHC staining of human liver using 17442-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Enoyl-CoA hydratase-1 regulates mTOR signaling and apoptosis by sensing nutrients.

Lipidomics reveals the potential mechanism of honokiol against adenine-induced chronic kidney disease

The serum acylcarnitines profile in epileptic children treated with valproic acid and the protective roles of peroxisome proliferator-activated receptor a activation in valproic acid-induced liver injury

Chaperonin counteracts diet-induced non-alcoholic fatty liver disease by aiding sirtuin 3 in the control of fatty acid oxidation

A HIF-1α inhibitor combined with palmitic acid and L-carnitine treatment can prevent the fat metabolic reprogramming under hypoxia and induce apoptosis in hepatocellular carcinoma cells