ACADM Monoclonal antibody
ACADM Monoclonal Antibody for WB, IF, IHC, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
Human, rat, pig
Applications
WB, IHC, IF-P, ELISA
Conjugate
Unconjugated
CloneNo.
1A11G6
Cat no : 67742-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | PC-3 cells, HSC-T6 cells, rat liver tissue, HeLa cells, HEK-293 cells, HepG2 cells |
Positive IHC detected in | human liver tissue, human heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF-P detected in | human liver cancer tissue |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:2000-1:10000 |
Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
WB | See 1 publications below |
Product Information
67742-1-Ig targets ACADM in WB, IHC, IF-P, ELISA applications and shows reactivity with Human, rat, pig samples.
Tested Reactivity | Human, rat, pig |
Cited Reactivity | rat |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Immunogen | ACADM fusion protein Ag30620 相同性解析による交差性が予測される生物種 |
Full Name | acyl-Coenzyme A dehydrogenase, C-4 to C-12 straight chain |
Calculated molecular weight | 421 aa, 47 kDa |
Observed molecular weight | 42 kDa |
GenBank accession number | BC005377 |
Gene symbol | ACADM |
Gene ID (NCBI) | 34 |
RRID | AB_2918511 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein G purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
ACADM, also named as MCAD, belongs to the acyl-CoA dehydrogenase family. This enzyme is specific for acyl chain lengths of 4 to 16. It catalyzes the reaction: Acyl-CoA + acceptor = 2,3-dehydroacyl-CoA + reduced acceptor. Defects in ACADM are the cause of medium-chain acyl-CoA dehydrogenase deficiency (MCAD deficiency). This protein can exsit as a dimer(PMID:8962055). This antibody is specific to ACADM.
Protocols
Product Specific Protocols | |
---|---|
WB protocol for ACADM antibody 67742-1-Ig | Download protocol |
IHC protocol for ACADM antibody 67742-1-Ig | Download protocol |
IF protocol for ACADM antibody 67742-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Int J Mol Sci High-Dose Fenofibrate Stimulates Multiple Cellular Stress Pathways in the Kidney of Old Rats |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Agata (Verified Customer) (04-16-2024) | The antibody used in WB gave a single clear band, of appropriate weight, with a strong signal, in rat kidney tissue homogenates - the antibody was excellent on every try.
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