Aggrecan Polyclonal antibody
Aggrecan Polyclonal Antibody for IHC, FC (Intra), ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (4)
Applications
IHC, IF, FC (Intra), ELISA
Conjugate
Unconjugated
260
Cat no : 13880-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive IHC detected in | human lung tissue, human cartilage tissue, human lung cancer tissue, mouse lung tissue, rat brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive FC (Intra) detected in | ATDC-5 cells |
Recommended dilution
Application | Dilution |
---|---|
Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
IHC | See 86 publications below |
IF | See 82 publications below |
Product Information
13880-1-AP targets Aggrecan in IHC, IF, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat, pig, rabbit, canine, zebrafish |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Aggrecan fusion protein Ag4985 相同性解析による交差性が予測される生物種 |
Full Name | aggrecan |
Calculated molecular weight | 250 kDa |
GenBank accession number | BC036445 |
Gene symbol | ACAN |
Gene ID (NCBI) | 176 |
RRID | AB_2722780 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Aggrecan (ACAN), also named as CSPCP, AGC1 and MSK16, belongs to the aggrecan/versican proteoglycan family (also including versican, brevican and neurocan). It is a major component of extracellular matrix of cartilaginous tissues. A major function of aggrecan is to resist compression in cartilage. It binds avidly to hyaluronic acid via an N-terminal globular region.
Protocols
Product Specific Protocols | |
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IHC protocol for Aggrecan antibody 13880-1-AP | Download protocol |
FC protocol for Aggrecan antibody 13880-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Sci Transl Med Single-cell atlas of human infrapatellar fat pad and synovium implicates APOE signaling in osteoarthritis pathology | ||
J Extracell Vesicles miR-23a-3p-abundant small extracellular vesicles released from Gelma/nanoclay hydrogel for cartilage regeneration. | ||
Biomaterials Injectable pathological microenvironment-responsive anti-inflammatory hydrogels for ameliorating intervertebral disc degeneration | ||
Cell Death Differ Developmental growth plate cartilage formation suppressed by artificial light at night via inhibiting BMAL1-driven collagen hydroxylation | ||
Nat Commun Mutant Huntingtin stalls ribosomes and represses protein synthesis in a cellular model of Huntington disease. |