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Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human lung cancer tissue using Aggrecan Monoclonal antibody (68350-1-Ig)

    IHC staining of human lung cancer using 68350-1-Ig (same clone as 68350-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 68350-1-Ig (ACAN antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68350-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using Aggrecan Monoclonal antibody (68350-1-Ig)

    IF Staining of HEK-293 using 68350-1-Ig (same clone as 68350-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using ACAN antibody (68350-1-Ig, Clone: 1G11B3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68350-1-PBS in a different storage buffer formulation.

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Aggrecan Monoclonal antibody, PBS Only

Aggrecan Monoclonal Antibody for IHC, IF/ICC, Indirect ELISA
Cat No. 68350-1-PBS
Clone No.1G11B3

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

IHC, IF/ICC, Indirect ELISA

Synonyms

ACAN, 1G11B3, Aggrecan core protein 2, Aggrecan 1, AGCAN

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68350-1-PBS targets Aggrecan in IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen Aggrecan fusion protein Ag4985 相同性解析による交差性が予測される生物種
Full Name aggrecan
Calculated molecular weight 250 kDa
GenBank accession numberBC036445
Gene Symbol ACAN
Gene ID (NCBI) 176
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP16112
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Aggrecan (ACAN), also named CSPCP, AGC1 and MSK16, belongs to the aggrecan/versican proteoglycan family (also including versican, brevican and neurocan). It is a major component of the extracellular matrix of cartilaginous tissues. A major function of aggrecan is to resist compression in cartilage. It binds avidly to hyaluronic acid via an N-terminal globular region.