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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ACAP1 Monoclonal antibody

ACAP1 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1B4C9

Cat no : 66596-1-Ig

Synonyms

ACAP1, Centaurin beta 1, CENTB1, Cnt b1, KIAA0050

Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using ACAP1 Monoclonal antibody (66596-1-Ig)

    WB analysis of Jurkat using 66596-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66596-1-Ig (ACAP1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HL-60 cells using ACAP1 Monoclonal antibody (66596-1-Ig)

    WB analysis of HL-60 using 66596-1-Ig

    HL-60 cells were subjected to SDS PAGE followed by western blot with 66596-1-Ig (ACAP1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using ACAP1 Monoclonal antibody (66596-1-Ig)

    IHC staining of human breast cancer using 66596-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66596-1-Ig (ACAP1 antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inJurkat cells, HL-60 cells, K-562 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:150-1:600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

66596-1-Ig targets ACAP1 in WB, IHC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Cited Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen ACAP1 fusion protein Ag14254 相同性解析による交差性が予測される生物種
Full Name ArfGAP with coiled-coil, ankyrin repeat and PH domains 1
Calculated molecular weight 740 aa, 82 kDa
Observed molecular weight 81 kDa
GenBank accession numberBC018543
Gene symbol ACAP1
Gene ID (NCBI) 9744
RRIDAB_2881956
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ACAP1, also named as Centaurin-beta-1, is a 740 amino acid protein, which is expressed Highly in lung and spleen. ACAP1 as a GTPase-activating protein (GAP) for ADP ribosylation factor 6 (ARF6) is required for clathrin-dependent export of proteins from recycling endosomes to trans-Golgi network and cell surface and required for regulated export of ITGB1 from recycling endosomes to the cell surface and ITGB1-dependent cell migration.

Protocols

Product Specific Protocols
WB protocol for ACAP1 antibody 66596-1-IgDownload protocol
IHC protocol for ACAP1 antibody 66596-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cancers (Basel)

ACAP1 Deficiency Predicts Inferior Immunotherapy Response in Solid Tumors

Authors - Qiyi Yi
View Article