• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of Jurkat cells using ACAP1 Recombinant antibody (82967-4-RR)

    WB analysis of Jurkat using 82967-4-RR (same clone as 82967-4-PBS)

    WB result of ACAP1 antibody (82967-4-RR; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ACAP1 transfected Jurkat cells. This data was developed using the same antibody clone with 82967-4-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using ACAP1 Recombinant antibody (82967-4-RR)

    WB analysis using 82967-4-RR (same clone as 82967-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82967-4-RR (ACAP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82967-4-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of Jurkat cells using ACAP1 Recombinant antibody (82967-4-RR)

    IF Staining of Jurkat using 82967-4-RR (same clone as 82967-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using ACAP1 antibody (82967-4-RR, Clone: 230370D5 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 82967-4-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of A431 cells using ACAP1 Recombinant antibody (82967-4-RR)

    FC experiment of A431 using 82967-4-RR (same clone as 82967-4-PBS)

    1x10^6 A431 cells were intracellularly stained with 0.4 ug ACAP1 Recombinant antibody (82967-4-RR, Clone:230370D5) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 82967-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82967-4-RR

    Affinity and Kinetic Characterization of 82967-4-RR

    Biolayer interferometry (BLl) kinetic assays of 82967-4-RR against Human ACAP1 were performed. The affinity constant is below 1 pM.

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

  • Featured Product
  • KD/KO Validated

ACAP1 Recombinant antibody, PBS Only

ACAP1 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 82967-4-PBS
Clone No.230370D5

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), Indirect ELISA

Synonyms

Cnt b1, CENTB1, Centaurin-beta-1, Centaurin beta 1, Arf-GAP with coiled-coil, ANK repeat and PH domain-containing protein 1

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

82967-4-PBS targets ACAP1 in WB, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen ACAP1 fusion protein Ag34309 相同性解析による交差性が予測される生物種
Full Name ArfGAP with coiled-coil, ankyrin repeat and PH domains 1
Calculated molecular weight 740 aa, 82 kDa
Observed molecular weight 82-85 kDa
GenBank accession numberBC018543
Gene Symbol ACAP1
Gene ID (NCBI) 9744
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ15027
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

ACAP1, also named as Centaurin-beta-1, is a 740 amino acid protein, which is expressed Highly in lung and spleen. ACAP1 as a GTPase-activating protein (GAP) for ADP ribosylation factor 6 (ARF6) is required for clathrin-dependent export of proteins from recycling endosomes to trans-Golgi network and cell surface and required for regulated export of ITGB1 from recycling endosomes to the cell surface and ITGB1-dependent cell migration.