Validation Data Gallery
Tested Applications
| Positive WB detected in | Jurkat cells, SH-SY5Y cells |
| Positive IF/ICC detected in | SH-SY5Y cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 3 publications below |
| IF | See 1 publications below |
Product Information
17570-1-AP targets ACAP3 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | ACAP3 fusion protein Ag11777 相同性解析による交差性が予測される生物種 |
| Full Name | ArfGAP with coiled-coil, ankyrin repeat and PH domains 3 |
| Calculated molecular weight | 834 aa, 92 kDa |
| Observed molecular weight | 85kda, 92 kDa |
| GenBank accession number | BC047001 |
| Gene Symbol | ACAP3 |
| Gene ID (NCBI) | 116983 |
| RRID | AB_2257668 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q96P50 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
ACAP3 (Arf-GAP with coiled-coil, ANK repeat and PH domain-containing protein 3) , also known as CENTB5, is a protein-coding gene that belongs to the ACAP family of GTPase-activating proteins (GAPs). ACAP3 is involved in neuronal migration in the developing cerebral cortex of mice. Knockdown of ACAP3 in the developing cortical neurons of mice in utero significantly abrogated neuronal migration in the cortical layer, ACAP3 is involved in neuronal migration in the developing cerebral cortex of mice. Knockdown of ACAP3 in the developing cortical neurons of mice in utero significantly abrogated neuronal migration in the cortical layer (PMID: 28919417),
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for ACAP3 antibody 17570-1-AP | Download protocol |
| IF protocol for ACAP3 antibody 17570-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Biochem J ACAP3 regulates neurite outgrowth through its GAP activity specific to Arf6 in mouse hippocampal neurons. | ||
Biochem Biophys Res Commun ACAP3, the GTPase-activating protein specific to the small GTPase Arf6, regulates neuronal migration in the developing cerebral cortex.
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