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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ACBD3 Polyclonal antibody

ACBD3 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 14096-1-AP

Synonyms

Golgi resident protein GCP60, Golgi phosphoprotein 1, Golgi complex-associated protein 1, GOCAP1, GCP60

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ACBD3 Polyclonal antibody (14096-1-AP)

    WB analysis using 14096-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14096-1-AP (ACBD3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse ovary tissue using ACBD3 Polyclonal antibody (14096-1-AP)

    WB analysis of mouse ovary using 14096-1-AP

    mouse ovary tissue were subjected to SDS PAGE followed by western blot with 14096-1-AP (ACBD3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse testis tissue using ACBD3 Polyclonal antibody (14096-1-AP)

    WB analysis of mouse testis using 14096-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 14096-1-AP (ACBD3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using ACBD3 Polyclonal antibody (14096-1-AP)

    IHC staining of mouse testis using 14096-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 14096-1-AP (ACBD3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using ACBD3 Polyclonal antibody (14096-1-AP)

    IHC staining of mouse testis using 14096-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 14096-1-AP (ACBD3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using ACBD3 Polyclonal antibody (14096-1-AP)

    IF Staining of HeLa using 14096-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 14096-1-AP (ACBD3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inHEK-293T cells, mouse ovary tissue, mouse testis tissue
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14096-1-AP targets ACBD3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ACBD3 fusion protein Ag5228 相同性解析による交差性が予測される生物種
Full Name acyl-Coenzyme A binding domain containing 3
Calculated molecular weight 528 aa, 61 kDa
Observed molecular weight 60-70 kDa
GenBank accession numberBC060792
Gene symbol ACBD3
Gene ID (NCBI) 64746
RRIDAB_2220062
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for ACBD3 antibody 14096-1-APDownload protocol
IHC protocol for ACBD3 antibody 14096-1-APDownload protocol
IF protocol for ACBD3 antibody 14096-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Biol Int

Golgi protein ACBD3 downregulation sensitizes cells to ferroptosis

Authors - Ying Qian
  • KD Validated
View Article