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Validation Data Gallery

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  • Western Blot (WB) analysis of human kidney tissue using ACBD4 Polyclonal antibody (20941-1-AP)

    WB analysis of human kidney using 20941-1-AP

    human kidney tissue were subjected to SDS PAGE followed by western blot with 20941-1-AP (ACBD4 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using ACBD4 Polyclonal antibody (20941-1-AP)

    IF Staining of MCF-7 using 20941-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using ACBD4 antibody (20941-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4).

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

ACBD4 Polyclonal antibody

ACBD4 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 20941-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IF/ICC, ELISA

Synonyms

HMFT0700, Acyl-CoA-binding domain-containing protein 4

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inhuman kidney tissue
Positive IF/ICC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

20941-1-AP targets ACBD4 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ACBD4 fusion protein Ag15045 相同性解析による交差性が予測される生物種
Full Name acyl-Coenzyme A binding domain containing 4
Calculated molecular weight 341 aa, 38 kDa
Observed molecular weight 30-38 kDa
GenBank accession numberBC029164
Gene Symbol ACBD4
Gene ID (NCBI) 79777
RRIDAB_2878769
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8NC06
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for ACBD4 antibody 20941-1-APDownload protocol
IF protocol for ACBD4 antibody 20941-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Nat Struct Mol Biol

TDP-43 aggregation induced by oxidative stress causes global mitochondrial imbalance in ALS.

Authors - Xinxin Zuo
View Article