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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ACC1 Recombinant antibody, PBS Only

ACC1 Recombinant Antibody for WB, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2I13

Cat no : 81656-1-PBS

Synonyms

ACACA, ACC-alpha, ACCA, ACC, ACAC

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ACC1 Recombinant antibody (81656-1-RR)

    WB analysis using 81656-1-RR (same clone as 81656-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 81656-1-RR (ACC1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81656-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HepG2 cells using ACC1 Recombinant antibody (81656-1-RR)

    IP experiment of HepG2 using 81656-1-RR (same clone as 81656-1-PBS)

    IP result of anti-ACC1 (IP:81656-1-RR, 4ug; Detection:81656-1-RR 1:2000) with HepG2 cells lysate 1640 ug. This data was developed using the same antibody clone with 81656-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 81656-1-RR

    Affinity and Kinetic Characterization of 81656-1-RR

    Biolayer interferometry (BLl) kinetic assays of 81656-1-RR against Human ACC1 were performed. The affinity constant is 2.28 nM.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

81656-1-PBS targets ACC1 in WB, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen ACC1 fusion protein Ag16452 相同性解析による交差性が予測される生物種
Full Name acetyl-Coenzyme A carboxylase alpha
Calculated molecular weight 2383 aa, 275 kDa
Observed molecular weight 250 kDa
GenBank accession numberBC137287
Gene symbol Acetyl-CoA Carboxylase 1
Gene ID (NCBI) 31
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

ACACA(Acetyl-CoA carboxylase 1, ACC), also named as ACAC, ACC1 and ACCA, belongs to the biotin containing enzyme family. It catalyzes the synthesis of malonyl-CoA, which is an intermediate substrate playing a pivotal role in the regulation of fatty acid metabolism and energy production. ACACA is involved in the biosynthesis of fatty acids, and malonyl-CoA produced is used as a building block to extend the chain length of fatty acids by fatty acid synthase (FAS)(PMID:19900410). It has 4 isoforms produced by alternative promoter usage with the molecular weight between 260 kDa and 270 kDa.