Validation Data Gallery
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Tested Applications
| Positive WB detected in | rat brain tissue |
| Positive IP detected in | mouse brain tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 2 publications below |
Product Information
17851-1-AP targets ASIC2 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | ASIC2 fusion protein Ag12301 相同性解析による交差性が予測される生物種 |
| Full Name | amiloride-sensitive cation channel 1, neuronal |
| Calculated molecular weight | 512 aa, 58 kDa |
| Observed molecular weight | 70 kDa |
| GenBank accession number | BC075043 |
| Gene Symbol | ASIC2 |
| Gene ID (NCBI) | 40 |
| RRID | AB_2878451 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q16515 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
ASIC2 (acid-sensing ion channel 2), also known as ACCN1 (amiloride-sensitive cation channel 1, neuronal), is a member of the degenerin/epithelial sodium channel (DEG/ENaC) superfamily. ASIC2 is widely expressed in the central nervous system (CNS), may playing a role in neurotransmission. It has two alternatively spliced variants, ASIC2a and ASIC2b.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for ASIC2 antibody 17851-1-AP | Download protocol |
| IP protocol for ASIC2 antibody 17851-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Aging (Albany NY) ASIC1 and ASIC3 mediate cellular senescence of human nucleus pulposus mesenchymal stem cells during intervertebral disc degeneration. | ||