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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ACE Recombinant antibody

ACE Recombinant Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

241763E3

Cat no : 84407-4-RR

Synonyms

DCP, Angiotensin-converting enzyme, soluble form, Angiotensin-converting enzyme, Angiotensin converting enzyme, ACE1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ACE Recombinant antibody (84407-4-RR)

    WB analysis using 84407-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84407-4-RR (ACE antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of rat lung tissue using ACE Recombinant antibody (84407-4-RR)

    IHC staining of rat lung using 84407-4-RR

    Immunohistochemical analysis of paraffin-embedded rat lung tissue slide using 84407-4-RR (ACE antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse lung tissue using ACE Recombinant antibody (84407-4-RR)

    IHC staining of mouse lung using 84407-4-RR

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 84407-4-RR (ACE antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse lung tissue using ACE Recombinant antibody (84407-4-RR)

    IHC staining of mouse lung using 84407-4-RR

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 84407-4-RR (ACE antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inmouse lung tissue, rat lung tissue
Positive IHC detected inmouse lung tissue, rat lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84407-4-RR targets ACE in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant protein 相同性解析による交差性が予測される生物種
Full Name angiotensin I converting enzyme (peptidyl-dipeptidase A) 1
Calculated molecular weight150kDa
Observed molecular weight170-180 kDa
GenBank accession numberNM_000789.4
Gene symbol ACE
Gene ID (NCBI) 1636
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ACE(Angiotensin-converting enzyme) is also named as DCP, DCP1 and belongs to the peptidase M2 family. It is involved in catalyzing the conversion of angiotensin I into a physiologically active peptide angiotensin II. ACE also has a glycosidase activity which releases GPI-anchored proteins from the membrane by cleaving the mannose linkage in the GPI moiety. It has 4 isoforms produced by alternative splicing. The full length protein has 15 glycosylation sites and it can show a molecular weight of 170 kDa(PMID:16804085).

Protocols

Product Specific Protocols
WB protocol for ACE antibody 84407-4-RRDownload protocol
IHC protocol for ACE antibody 84407-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols