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  • Featured Product
  • KD/KO Validated

ACE2 Polyclonal antibody

ACE2 Polyclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

Cat no : 28868-1-AP

Synonyms

Angiotensin-converting enzyme homolog, Angiotensin-converting enzyme 2, Angiotensin converting enzyme 2, ACE-related carboxypeptidase, ACEH

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ACE2 Polyclonal antibody (28868-1-AP)

    WB analysis using 28868-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28868-1-AP (ACE2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using ACE2 Polyclonal antibody (28868-1-AP)

    WB analysis of HeLa using 28868-1-AP

    WB result of ACE2 antibody (28868-1-AP; 1:1200; incubated at room temperature for 1.5 hours) with sh-Control and sh-ACE2 transfected HeLa cells.

  • Immunohistochemistry (IHC) staining of human kidney tissue using ACE2 Polyclonal antibody (28868-1-AP)

    IHC staining of human kidney using 28868-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 28868-1-AP (ACE2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human kidney tissue using ACE2 Polyclonal antibody (28868-1-AP)

    IHC staining of human kidney using 28868-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 28868-1-AP (ACE2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using ACE2 Polyclonal antibody (28868-1-AP)

    IHC staining of human colon cancer using 28868-1-AP

    Immunohistochemical analysis of paraffin-embedded colon cancer slide using 28868-1-AP (ACE2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human small intestine tissue using ACE2 Polyclonal antibody (28868-1-AP)

    IHC staining of human small intestine using 28868-1-AP

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 28868-1-AP (ACE2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human small intestine tissue using ACE2 Polyclonal antibody (28868-1-AP)

    IHC staining of human small intestine using 28868-1-AP

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 28868-1-AP (ACE2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse kidney tissue using ACE2 Polyclonal antibody (28868-1-AP)

    IF Staining of mouse kidney using 28868-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse kidney tissue using ACE2 antibody (28868-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse kidney tissue using ACE2 Polyclonal antibody (28868-1-AP)

    IF Staining of mouse kidney using 28868-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse kidney tissue using ACE2 antibody (28868-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inmouse kidney tissue, HeLa cells, mouse testis tissue, rat testis tissue
Positive IHC detected inhuman kidney tissue, human colon cancer tissue, human small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse kidney tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

28868-1-AP targets ACE2 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ACE2 fusion protein Ag30683 相同性解析による交差性が予測される生物種
Full Name angiotensin I converting enzyme (peptidyl-dipeptidase A) 2
Calculated molecular weight 805 aa, 92 kDa
Observed molecular weight92, 120 kDa
GenBank accession numberBC048094
Gene symbol ACE2
Gene ID (NCBI) 59272
RRIDAB_2881224
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ACE2 (Angiotensin-converting enzyme 2), also named as ACEH, is a zinc metalloprotease of the ACE family and a critical regulator of the reninangiotensin system. ACE2 has a more restricted tissue distribution than ACE, being found predominantly in the heart, kidneys, and testes although low levels have been detected in a variety of tissues (PMID:15983030). ACE2 has been shown to be a functional receptor of the human coronaviruses SARS-CoV and SARS-CoV-2 (PMID: 32142651). The expression level and expression pattern of human ACE2 in different tissues might be critical for the susceptibility, symptoms, and outcome of 2019-nCoV/SARS-CoV-2 infection (PMID: 32133153). It can be used as a potential therapeutic target of SARS-CoV-2 (PMID: 32125455). The calculated molecular weight of ACE2 is 92kDa but it migrates to 120kDa due to N-glycosylation (PMID:16166094). Sometimes, several cleaved fragments can also be detected as 75kDa, 50 kDa or 37kDa (PMID: 29561187, 22009550, 30759273). It has 2 isoforms produced by alternative splicing. This antibody is specific to ACE2. The location of ACE2 is membrane and cytoplasm, however it accumulates in the nucleus during the mitosis (PMID: 1730413/PMID: 18292088). 28868-1-AP is specific for ACE2, and its antigen region is 30-356aa.

Protocols

Product Specific Protocols
WB protocol for ACE2 antibody 28868-1-APDownload protocol
IHC protocol for ACE2 antibody 28868-1-APDownload protocol
IF protocol for ACE2 antibody 28868-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols