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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ACE2 Polyclonal antibody (31518-1-AP)

    WB analysis using 31518-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31518-1-AP (ACE2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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ACE2 Polyclonal antibody

ACE2 Polyclonal Antibody for WB, ELISA
Cat No. 31518-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Positive WB detected inmouse kidney tissue, mouse small intestine tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

31518-1-AP targets ACE2 in WB, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Recombinant protein 相同性解析による交差性が予測される生物種
Full Name angiotensin I converting enzyme (peptidyl-dipeptidase A) 2
Calculated molecular weight 805 aa, 92 kDa
Observed molecular weight120 kDa
GenBank accession numberBC048094
Gene Symbol ACE2
Gene ID (NCBI) 59272
RRIDAB_3670016
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDQ9BYF1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Angiotensin-converting enzyme 2 (ACE2), a homolog of angiotensin-converting enzyme 1 (ACE1), was identified in 2000. ACE2 provides protection against several chronic diseases, including cardiovascular diseases, lung injury, and diabetes. Besides chronic diseases, ACE2 plays a crucial role in severe acute respiratory syndrome coronavirus (SARS-CoV) infection and severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection (hereafter named COVID-19). ACE2 is the host receptor for SARS-CoV and SARS-CoV-2. (PMID: 37608279)

Protocols

Product Specific Protocols
WB protocol for ACE2 antibody 31518-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols