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  • Featured Product
  • KD/KO Validated

ACE2 Monoclonal antibody, PBS Only

ACE2 Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB, IHC, IF-P, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2F12A4

Cat no : 66699-1-PBS

Synonyms

Angiotensin-converting enzyme 2, Angiotensin converting enzyme 2, ACE-related carboxypeptidase, ACEH, ACE related carboxypeptidase

Validation Data Gallery

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  • Western Blot (WB) analysis of human testis tissue using ACE2 Monoclonal antibody (66699-1-Ig)

    WB analysis of human testis using 66699-1-Ig (same clone as 66699-1-PBS)

    Untreated and PNGase F-treated lysates of human testis tissue were subjected to SDS PAGE followed by western blot with 66699-1-Ig (ACE2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. PNGase F was obtained from Atagenix (cat.NO. ata808). This data was developed using the same antibody clone with 66699-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human testis tissue using ACE2 Monoclonal antibody (66699-1-Ig)

    WB analysis of human testis using 66699-1-Ig (same clone as 66699-1-PBS)

    human testis tissue were subjected to SDS PAGE followed by western blot with 66699-1-Ig (ACE2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66699-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human skeletal muscle tissue using ACE2 Monoclonal antibody (66699-1-Ig)

    WB analysis of human skeletal muscle using 66699-1-Ig (same clone as 66699-1-PBS)

    human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 66699-1-Ig (ACE2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66699-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human small intestine tissue using ACE2 Monoclonal antibody (66699-1-Ig)

    IHC staining of human small intestine using 66699-1-Ig (same clone as 66699-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 66699-1-Ig (ACE2 antibody) at dilution of 1:2000 (under 10x lens).. This data was developed using the same antibody clone with 66699-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human small intestine tissue using ACE2 Monoclonal antibody (66699-1-Ig)

    IHC staining of human small intestine using 66699-1-Ig (same clone as 66699-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 66699-1-Ig (ACE2 antibody) at dilution of 1:2000 (under 40x lens).. This data was developed using the same antibody clone with 66699-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using ACE2 Monoclonal antibody (66699-1-Ig)

    IHC staining of human colon cancer using 66699-1-Ig (same clone as 66699-1-PBS)

    Immunohistochemical analysis of paraffin-embedded colon cancer slide using 66699-1-Ig (ACE2 antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66699-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using ACE2 Monoclonal antibody (66699-1-Ig)

    IHC staining of human testis using 66699-1-Ig (same clone as 66699-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 66699-1-Ig (ACE2 antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66699-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using ACE2 Monoclonal antibody (66699-1-Ig)

    IHC staining of human testis using 66699-1-Ig (same clone as 66699-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 66699-1-Ig (ACE2 antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66699-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using ACE2 Monoclonal antibody (66699-1-Ig)

    IHC staining of human kidney using 66699-1-Ig (same clone as 66699-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66699-1-Ig (ACE2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66699-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using ACE2 Monoclonal antibody (66699-1-Ig)

    IHC staining of human kidney using 66699-1-Ig (same clone as 66699-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66699-1-Ig (ACE2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66699-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using ACE2 Monoclonal antibody (66699-1-Ig)

    IHC staining of human colon using 66699-1-Ig (same clone as 66699-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66699-1-Ig (ACE2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66699-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using ACE2 Monoclonal antibody (66699-1-Ig)

    IHC staining of human colon using 66699-1-Ig (same clone as 66699-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66699-1-Ig (ACE2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66699-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human kidney tissue using ACE2 Monoclonal antibody (66699-1-Ig)

    IF Staining of human kidney using 66699-1-Ig (same clone as 66699-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using ACE2 antibody (66699-1-Ig, Clone: 2F12A4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66699-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human kidney tissue using ACE2 Monoclonal antibody (66699-1-Ig)

    IF Staining of human kidney using 66699-1-Ig (same clone as 66699-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using ACE2 antibody (66699-1-Ig, Clone: 2F12A4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66699-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse heart tissue using ACE2 Monoclonal antibody (66699-1-Ig)

    IF Staining of mouse heart using 66699-1-Ig (same clone as 66699-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using ACE2 antibody (66699-1-Ig, Clone: 2F12A4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66699-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse testis tissue using ACE2 Monoclonal antibody (66699-1-Ig)

    IF Staining of mouse testis using 66699-1-Ig (same clone as 66699-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse testis tissue using ACE2 antibody (66699-1-Ig, Clone: 2F12A4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66699-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse kidney tissue using ACE2 Monoclonal antibody (66699-1-Ig)

    IF Staining of mouse kidney using 66699-1-Ig (same clone as 66699-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse kidney tissue using ACE2 antibody (66699-1-Ig, Clone: 2F12A4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66699-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse kidney tissue using ACE2 Monoclonal antibody (66699-1-Ig)

    IF Staining of mouse kidney using 66699-1-Ig (same clone as 66699-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse kidney tissue using ACE2 antibody (66699-1-Ig, Clone: 2F12A4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66699-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66699-1-PBS targets ACE2 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen ACE2 fusion protein Ag15554 相同性解析による交差性が予測される生物種
Full Name angiotensin I converting enzyme (peptidyl-dipeptidase A) 2
Calculated molecular weight 805 aa, 92 kDa
Observed molecular weight 120 kDa, 92 kDa
GenBank accession numberBC048094
Gene symbol ACE2
Gene ID (NCBI) 59272
RRIDAB_2882052
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

ACE2 (Angiotensin-converting enzyme 2), also named as ACEH, is a zinc metalloprotease of the ACE family and a critical regulator of the reninangiotensin system. ACE2 has a more restricted tissue distribution than ACE, being found predominantly in the heart, kidneys, and testes although low levels have been detected in a variety of tissues (PMID:15983030). ACE2 has been shown to be a functional receptor of the human coronaviruses SARS-CoV and SARS-CoV-2 (PMID: 32142651). The expression level and expression pattern of human ACE2 in different tissues might be critical for the susceptibility, symptoms, and outcome of 2019-nCoV/SARS-CoV-2 infection (PMID: 32133153). It can be used as a potential therapeutic target of SARS-CoV-2 (PMID: 32125455). The calculated molecular weight of ACE2 is 92kDa but it migrates to 120kDa due to N-glycosylation (PMID:16166094). Sometimes, several cleaved fragments can also be detected as 75kDa, 50 kDa or 37kDa (PMID: 29561187, 22009550, 30759273). It has 2 isoforms produced by alternative splicing. This antibody is specific to ACE2. The location of ACE2 is membrane and cytoplasm, however it accumulates in the nucleus during the mitosis (PMID: 1730413/PMID: 18292088).