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  • Featured Product
  • KD/KO Validated

ACK1 Polyclonal antibody, PBS Only

ACK1 Polyclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 14304-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of A549 cells using ACK1 Polyclonal antibody (14304-1-AP)

    WB analysis of A549 using 14304-1-AP (same clone as 14304-1-PBS)

    WB result of ACK1 antibody (14304-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ACK1 transfected A549 cells. This data was developed using the same antibody clone with 14304-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of SH-SY5Y cells using ACK1 Polyclonal antibody (14304-1-AP)

    WB analysis of SH-SY5Y using 14304-1-AP (same clone as 14304-1-PBS)

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 14304-1-AP (ACK1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14304-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HT-1080 cells using ACK1 Polyclonal antibody (14304-1-AP)

    WB analysis of HT-1080 using 14304-1-AP (same clone as 14304-1-PBS)

    HT-1080 cells were subjected to SDS PAGE followed by western blot with 14304-1-AP (ACK1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14304-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A431 cells using ACK1 Polyclonal antibody (14304-1-AP)

    WB analysis of A431 using 14304-1-AP (same clone as 14304-1-PBS)

    A431 cells were subjected to SDS PAGE followed by western blot with 14304-1-AP (ACK1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14304-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse brain tissue using ACK1 Polyclonal antibody (14304-1-AP)

    WB analysis of mouse brain using 14304-1-AP (same clone as 14304-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 14304-1-AP (ACK1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14304-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using ACK1 Polyclonal antibody (14304-1-AP)

    WB analysis of HeLa using 14304-1-AP (same clone as 14304-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 14304-1-AP (ACK1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14304-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using ACK1 Polyclonal antibody (14304-1-AP)

    WB analysis of HepG2 using 14304-1-AP (same clone as 14304-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 14304-1-AP (ACK1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14304-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using ACK1 Polyclonal antibody (14304-1-AP)

    IHC staining of human breast cancer using 14304-1-AP (same clone as 14304-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 14304-1-AP (ACK1 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 14304-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using ACK1 Polyclonal antibody (14304-1-AP)

    IHC staining of human breast cancer using 14304-1-AP (same clone as 14304-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 14304-1-AP (ACK1 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 14304-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

14304-1-PBS targets ACK1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ACK1 fusion protein Ag5419 相同性解析による交差性が予測される生物種
Full Name tyrosine kinase, non-receptor, 2
Calculated molecular weight 115 kDa
Observed molecular weight 70 kDa
GenBank accession numberBC028164
Gene symbol TNK2
Gene ID (NCBI) 10188
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

TNK2, also named ACK1, belongs to the protein kinase superfamily and Tyr protein kinase family. It is a non-receptor tyrosine-protein and serine/threonine-protein kinase that is implicated in cell spreading and migration, cell survival, and cell proliferation. TNK2 transduces extracellular signals to cytosolic and nuclear effectors. It is a downstream effector of CDC42 which mediates CDC42-dependent cell migration via phosphorylation of BCAR1. TNK2 confers metastatic properties on cancer cells by negatively regulating tumor suppressors such as WWOX and positively regulating pro-survival factors such as AKT1 and AR. The 60-70kd band is the isoform 2 of TNK2. Isoform 1 and 3 is about 120-125kd.