Validation Data Gallery

Filter: AllWBIPIHCIF/ICCFC (Intra)

WB analysis of A549 using 15421-1-AP
WB result of ACLY antibody (15421-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ACLY transfected A549 cells.
WB analysis using 15421-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 15421-1-AP (ACLY antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
WB analysis using 15421-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 15421-1-AP (ATP citrate lyase antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis using 15421-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 15421-1-AP (ACLY antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
WB analysis using 15421-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 15421-1-AP (ATP citrate lyase antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of HeLa using 15421-1-AP
IP result of anti-ACLY (IP:15421-1-AP, 4ug; Detection:15421-1-AP 1:8000) with HeLa cells lysate 1320 ug.
IHC staining of human prostate cancer using 15421-1-AP
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 15421-1-AP (ACLY antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human prostate cancer using 15421-1-AP
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 15421-1-AP (ACLY antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of A549 using 15421-1-AP
Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using ACLY antibody (15421-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).
FC experiment of HepG2 using 15421-1-AP
1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human ACLY (15421-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Featured Product
KD/KO Validated

ACLY Polyclonal antibody

ACLY Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Cat No. 15421-1-AP

Publications(93)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA

Synonyms

ATP citrate lyase, ACL, ATP-citrate, ATP-citrate synthase, EC:2.3.3.8

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488 CoraLite® Plus 555 CoraLite® Plus 647

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (93)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	A549 cells, HeLa cells, L02 cells, Jurkat cells, mouse brain tissue, rat brain tissue, K-562 cells, MCF-7 cells, mouse liver tissue, rat liver tissue
Positive IP detected in	HeLa cells
Positive IHC detected in	human prostate cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	A549 cells
Positive FC (Intra) detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:5000-1:50000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 18 publications below
WB	See 88 publications below
IHC	See 7 publications below
IF	See 11 publications below
IP	See 4 publications below
CoIP	See 4 publications below

Product Information

15421-1-AP targets ACLY in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat, chicken
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag7709 Product name: Source: e coli.-derived, PGEX-4T Tag: GST Domain: of Sequence: 相同性解析による交差性が予測される生物種
Full Name	ATP citrate lyase
Calculated molecular weight	121 kDa
Observed molecular weight	120 kDa
GenBank accession number	BC006195
Gene Symbol	ACLY
Gene ID (NCBI)	47
RRID	AB_2223741
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P53396
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

ACLY(ATP-citrate synthase) is also named as ACL. It is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA. ACLY serves as not only a target in oxygenated cells for suppression of lipid synthesis and histone acetylation, but also as a susceptible target in hypoxic cells to restore inhibition of glycolysis. In nonsmall cell lung carcinoma and hepatocellular carcinoma, ACLY is overexpressed compared with normal parenchyma suggesting that ACLY may represent a common target among highly malignant tumors(PMID:19795461). This protein has 2 isoforms produced by alternative splicing.

Protocols

Product Specific Protocols
WB protocol for ACLY antibody 15421-1-AP	Download protocol
IHC protocol for ACLY antibody 15421-1-AP	Download protocol
IF protocol for ACLY antibody 15421-1-AP	Download protocol
IP protocol for ACLY antibody 15421-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB	Nature Dietary fructose feeds hepatic lipogenesis via microbiota-derived acetate. Authors - Steven Zhao View Article
mouse	WB,IF	Nature Acetyl-CoA synthetase regulates histone acetylation and hippocampal memory. Authors - Philipp Mews View Article
mouse,human	WB	Cell Acetate Production from Glucose and Coupling to Mitochondrial Metabolism in Mammals. Authors - Xiaojing Liu KO Validated View Article
mouse	WB	Cell Metab High dietary fructose promotes hepatocellular carcinoma progression by enhancing O-GlcNAcylation via microbiota-derived acetate Authors - Peng Zhou View Article
mouse	WB	Mol Cell Quantitative subcellular acyl-CoA analysis reveals distinct nuclear metabolism and isoleucine-dependent histone propionylation Authors - Sophie Trefely KO Validated View Article
human	WB	Mol Cell Nuclear Acetyl-CoA Production by ACLY Promotes Homologous Recombination. Authors - Sharanya Sivanand KD Validated View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of A549 using 15421-1-AP

WB analysis using 15421-1-AP

WB analysis using 15421-1-AP

WB analysis using 15421-1-AP

WB analysis using 15421-1-AP

IP experiment of HeLa using 15421-1-AP

IHC staining of human prostate cancer using 15421-1-AP

IHC staining of human prostate cancer using 15421-1-AP

IF Staining of A549 using 15421-1-AP

FC experiment of HepG2 using 15421-1-AP

ACLY Polyclonal antibody

ACLY Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Dietary fructose feeds hepatic lipogenesis via microbiota-derived acetate.

Acetyl-CoA synthetase regulates histone acetylation and hippocampal memory.

Acetate Production from Glucose and Coupling to Mitochondrial Metabolism in Mammals.

High dietary fructose promotes hepatocellular carcinoma progression by enhancing O-GlcNAcylation via microbiota-derived acetate

Quantitative subcellular acyl-CoA analysis reveals distinct nuclear metabolism and isoleucine-dependent histone propionylation

Nuclear Acetyl-CoA Production by ACLY Promotes Homologous Recombination.