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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ACLY Monoclonal antibody, PBS Only

ACLY Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgM

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1E10D5

Cat no : 67166-1-PBS

Synonyms

ACL, ACLY, ATP citrate (pro S ) lyase, ATP citrate lyase, ATP citrate synthase, ATPCL, Citrate cleavage enzyme, CLATP

Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using ACLY Monoclonal antibody (67166-1-Ig)

    WB analysis of Jurkat using 67166-1-Ig (same clone as 67166-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 67166-1-Ig (ACLY antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67166-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HSC-T6 cells using ACLY Monoclonal antibody (67166-1-Ig)

    WB analysis of HSC-T6 using 67166-1-Ig (same clone as 67166-1-PBS)

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67166-1-Ig (ACLY antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67166-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of MCF-7 cells using ACLY Monoclonal antibody (67166-1-Ig)

    WB analysis of MCF-7 using 67166-1-Ig (same clone as 67166-1-PBS)

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 67166-1-Ig (ACLY antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67166-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Jurkat cells using ACLY Monoclonal antibody (67166-1-Ig)

    WB analysis of Jurkat using 67166-1-Ig (same clone as 67166-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 67166-1-Ig (ACLY antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67166-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using ACLY Monoclonal antibody (67166-1-Ig)

    IHC staining of human prostate cancer using 67166-1-Ig (same clone as 67166-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 67166-1-Ig (ACLY antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67166-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using ACLY Monoclonal antibody (67166-1-Ig)

    IHC staining of human prostate cancer using 67166-1-Ig (same clone as 67166-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 67166-1-Ig (ACLY antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67166-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using ACLY Monoclonal antibody (67166-1-Ig)

    IF Staining of A549 using 67166-1-Ig (same clone as 67166-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using ACLY antibody (67166-1-Ig, Clone: 1E10D5 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67166-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67166-1-PBS targets ACLY in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Mouse / IgM
Class Monoclonal
Type Antibody
Immunogen ACLY fusion protein Ag7709 相同性解析による交差性が予測される生物種
Full Name ATP citrate lyase
Calculated molecular weight 121 kDa
Observed molecular weight 120 kDa
GenBank accession numberBC006195
Gene symbol ACLY
Gene ID (NCBI) 47
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

ACLY(ATP-citrate synthase) is also named as ACL. It is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA. ACLY serves as not only a target in oxygenated cells for suppression of lipid synthesis and histone acetylation, but also as a susceptible target in hypoxic cells to restore inhibition of glycolysis. In nonsmall cell lung carcinoma and hepatocellular carcinoma, ACLY is overexpressed compared with normal parenchyma suggesting that ACLY may represent a common target among highly malignant tumors(PMID:19795461). This protein has 2 isoforms produced by alternative splicing.