Validation Data Gallery
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Tested Applications
| Positive WB detected in | HeLa cells, mouse liver tissue, rat liver tissue |
| Positive IP detected in | HepG2 cells |
| Positive IHC detected in | human stomach tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse liver tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 10 publications below |
| IHC | See 2 publications below |
| IP | See 1 publications below |
Product Information
12406-1-AP targets Aconitase 1 in WB, IHC, IF-P, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Aconitase 1 fusion protein Ag3083 相同性解析による交差性が予測される生物種 |
| Full Name | aconitase 1, soluble |
| Calculated molecular weight | 889 aa, 98 kDa |
| Observed molecular weight | 98 kDa |
| GenBank accession number | BC018103 |
| Gene Symbol | Aconitase 1 |
| Gene ID (NCBI) | 48 |
| RRID | AB_10642942 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P21399 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Mammalian cells possess two distinct isoforms of aconitase encoded by different genes: Aconitase 1 [ACO1; cytosolic aconitase (c-aconitase)] encoded by ACO1 and Aconitase 2 [ACO2; mitochondrial aconitase (m-aconitase)] encoded by ACO2. In addition to the enzymatic roles in the cytoplasm, ACO1 also is known to bind to mRNAs of specific genes and maintain iron homeostasis.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Aconitase 1 antibody 12406-1-AP | Download protocol |
| IHC protocol for Aconitase 1 antibody 12406-1-AP | Download protocol |
| IF protocol for Aconitase 1 antibody 12406-1-AP | Download protocol |
| IP protocol for Aconitase 1 antibody 12406-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
iScience Asparagine starvation suppresses histone demethylation through iron depletion
| ||
Biochim Biophys Acta Mol Basis Dis Iron deposition-induced ferroptosis in alveolar type II cells promotes the development of pulmonary fibrosis. | ||
Cell Biosci Mutant huntingtin induces iron overload via up-regulating IRP1 in Huntington's disease. | ||
Drug Des Devel Ther Curcumin induces apoptosis and protective autophagy in castration-resistant prostate cancer cells through iron chelation. | ||
Biomolecules Carbamoyl-Phosphate Synthase 1 as a Novel Target of Phomoxanthone A, a Bioactive Fungal Metabolite. | ||
JOJ Ophthalmol Mitophagy, Ferritinophagy and Ferroptosis in Retinal Pigment Epithelial Cells Under High Glucose Conditions: Implications for Diabetic Retinopathy and Age-Related Retinal Diseases. |