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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ACOT11 Polyclonal antibody (10776-1-AP)

    WB analysis using 10776-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10776-1-AP (ACOT11 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using ACOT11 Polyclonal antibody (10776-1-AP)

    IHC staining of human prostate cancer using 10776-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 10776-1-AP (ACOT11 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using ACOT11 Polyclonal antibody (10776-1-AP)

    IHC staining of human prostate cancer using 10776-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10776-1-AP (ACOT11 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using ACOT11 Polyclonal antibody (10776-1-AP)

    IF Staining of A431 using 10776-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed A431 cells using 10776-1-AP (ACOT11 antibody), at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using ACOT11 Polyclonal antibody (10776-1-AP)

    IF Staining of U2OS using 10776-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using ACOT11 antibody (10776-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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ACOT11 Polyclonal antibody

ACOT11 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 10776-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

BFIT, Adipose-associated thioesterase, Acyl-coenzyme A thioesterase 11, Acyl-CoA thioesterase 11, Acyl-CoA thioester hydrolase 11

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inDU 145 cells, PC-3 cells
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells, U2OS cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

10776-1-AP targets ACOT11 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ACOT11 fusion protein Ag1176 相同性解析による交差性が予測される生物種
Full Name acyl-CoA thioesterase 11
Calculated molecular weight68.4 kDa
Observed molecular weight68-70 kDa
GenBank accession numberBC001517
Gene Symbol ACOT11
Gene ID (NCBI) 26027
RRIDAB_2257798
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8WXI4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ACOT11(Acyl-coenzyme A thioesterase 11) is also named as BFIT, KIAA0707, THEA.BFIT is mapped to syntenic regions of chromosomes 1 (human) and 4 (mouse) associated with body fatness and diet-induced obesity, potentially linking a deficit of BFIT activity with exacerbation of these traits and supports the transition of this tissue towards increased metabolic activity, probably through alteration of intracellular fatty acyl-CoA concentration(PMID:11696000).

Protocols

Product Specific Protocols
WB protocol for ACOT11 antibody 10776-1-APDownload protocol
IHC protocol for ACOT11 antibody 10776-1-APDownload protocol
IF protocol for ACOT11 antibody 10776-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Thorac Dis

Establishment and validation of a prognostic model for idiopathic pulmonary fibrosis based on mitochondrial-related genes

Authors - Xuewen Wang
View Article