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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ACOT12 Polyclonal antibody

ACOT12 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 17871-1-AP

Synonyms

CACH, Acyl-coenzyme A thioesterase 12, Acyl-CoA thioesterase 12, Acyl-CoA thioester hydrolase 12, ACOT 12

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ACOT12 Polyclonal antibody (17871-1-AP)

    WB analysis using 17871-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17871-1-AP (ACOT12 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, HuH-7 cells, rat liver tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17871-1-AP targets ACOT12 in WB, ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ACOT12 fusion protein Ag12355 相同性解析による交差性が予測される生物種
Full Name acyl-CoA thioesterase 12
Calculated molecular weight 555 aa, 62 kDa
Observed molecular weight 70 kDa
GenBank accession numberBC075011
Gene symbol ACOT12
Gene ID (NCBI) 134526
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ACOT12 is an acyl-coenzyme A thioesterase that hydrolyzes acyl-coenzyme A (acyl-CoA) to free fatty acids and coenzyme A, thereby regulating intracellular levels of acyl-CoA and free fatty acids. It plays a key role in lipid metabolism and is mainly expressed in the kidney, liver, and intestine. ACOT12 plays an important role in a variety of diseases, including renal fibrosis, nonalcoholic fatty liver disease (NAFLD), and hepatocellular carcinoma. In renal fibrosis, downregulation of ACOT12 expression leads to lipid accumulation and exacerbates the fibrotic process. Restoration of its expression ameliorates renal fibrosis, suggesting that ACOT12 is a potential therapeutic target.

Protocols

Product Specific Protocols
WB protocol for ACOT12 antibody 17871-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols