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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ACOT4 Polyclonal antibody

ACOT4 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 17316-1-AP

Synonyms

ACOT4, acyl CoA thioesterase 4, Acyl coenzyme A thioesterase 4, PTE 2b, PTE Ib, PTE1B, PTE2B, PTEIB

Validation Data Gallery

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  • Western Blot (WB) analysis of L02 cells using ACOT4 Polyclonal antibody (17316-1-AP)

    WB analysis of L02 using 17316-1-AP

    L02 cells were subjected to SDS PAGE followed by western blot with 17316-1-AP (ACOT4 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using ACOT4 Polyclonal antibody (17316-1-AP)

    WB analysis using 17316-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17316-1-AP (ACOT4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using ACOT4 Polyclonal antibody (17316-1-AP)

    IHC staining of mouse kidney using 17316-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 17316-1-AP (ACOT4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using ACOT4 Polyclonal antibody (17316-1-AP)

    IHC staining of mouse kidney using 17316-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 17316-1-AP (ACOT4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using ACOT4 Polyclonal antibody (17316-1-AP)

    IF Staining of A549 using 17316-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using ACOT4 antibody (17316-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inHepG2 cells, L02 cells
Positive IHC detected inmouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17316-1-AP targets ACOT4 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ACOT4 fusion protein Ag11286 相同性解析による交差性が予測される生物種
Full Name acyl-CoA thioesterase 4
Calculated molecular weight 421 aa, 46 kDa
Observed molecular weight 46 kDa
GenBank accession numberBC031799
Gene symbol ACOT4
Gene ID (NCBI) 122970
RRIDAB_3085521
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ACOT4, also known as PET2B and PTEIB, is an acyl-CoA thioesterases which catalyze the hydrolysis of acyl-CoAs to the free fatty acid and coenzyme A (CoASH), regulating their respective intracellular levels. ACOT4 has the strongest expression in liver and kidney and weaker expression in placenta, heart, and muscle(PMID: 16940157).

Protocols

Product Specific Protocols
WB protocol for ACOT4 antibody 17316-1-APDownload protocol
IHC protocol for ACOT4 antibody 17316-1-APDownload protocol
IF protocol for ACOT4 antibody 17316-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols